高灵敏和高特异性生物嗅觉传感系统研究  

A Study of High-Sensitive and Specific Olfactory Biosensing System

作　　者：庄柳静[1] 郭添添[1] 胡宁[1] 许科帝[1,2] 郑筱祥[1,2] 王平[1] 

机构地区：[1]浙江大学生物医学工程教育部重点实验室,杭州310027 [2]浙江大学求是高等研究院,杭州310027

出　　处：《电子科技大学学报》2014年第6期939-942,共4页Journal of University of Electronic Science and Technology of China

基　　金：国家自然科学基金(81027003);国家973项目(2009CB320303)

摘　　要：利用微电极植入技术,提出了一种新型的生物嗅觉传感系统,将多通道植入式微电极包埋于大鼠嗅球中,提取出嗅球神经元的气味响应信息。通过分析神经元响应活动从而进行气味检测与区分,发现该系统能够长期稳定地用于气味检测,且灵敏度达到10?10 mol/L。在单分子气味或自然气味检测中,该系统都具有很高的特异性。因此,该系统在实际气味检测(如肺癌呼出气体标志物检测、食品新鲜度检测、爆炸物搜索等)领域具有广泛的应用前景。Mammalian olfactory system can accurately identify odorants with high specificity and sensitivity. Taking advantage of multiple microelectrode implant technology, we developed a novel in vivo biosensing system for odor detection. The odorant information from conscious rats’ olfactory bulb （OB） is extracted by using microelectrode array. High-quality mitral/tufted （M/T） cell activity evoked by odorants could be obtained for at least three weeks. The responses of M/T cell carry sufficient information to discriminate presented odorants. In addition, we found the concentration detection limit of rat is below 10-10 mol/L for carvone. These results demonstrate that in vivo biosensing system has the characteristics of high sensitivity, continuous recording, and specificity, which presents a promising platform for specific trace odorant detection in many fields.

关 键 词：植入式电极 生物嗅觉传感系统 哺乳动物 气体检测 嗅球 

分 类 号：Q424[生物学—神经生物学]