Aldehyde-Sodium Alginate and Amino-Gelatin Preparation as Soft Tissue Adhesive  

作　　者：袁柳 耿晓华 李家俊 孙彬彬 莫秀梅 

机构地区：[1]State Key Laboratory for Modification of Chemical Fibers and Polymer Materials,College of Materials Science and Engineering,Donghua University [2]Biomaterials and Tissue Engineering Laboratory,College of Chemistry,Chemical Engineering and Biotechnology,Donghua University

出　　处：《Journal of Donghua University(English Edition)》2014年第4期503-506,共4页东华大学学报（英文版）

基　　金：National Natural Science Foundations of China(Nos.30973105,31271035);Shanghai Committee of Science and Technology of Nano Special,China(No.11nm0506200);Ph.D.Programs Foundation of Ministry of Education of China(No.20130075110005)

摘　　要：Sodium alginate and gelatin are both remarkable natural biomaterials; they all have been extensively applied in tissue engineering and other relative fields,due to their low price and good biocompatibility. In this paper,we oxidized sodium alginate with sodium periodate to convert 1,2-hydroxyl groups into aldehyde groups to get aldehyde-sodium alginate( A-SA). Gelatin was modified with ethylenediamine( ED) in the presence of water-soluble1-ethyl-3( 3-dimethylaminopropyl) carbodiimide( EDC) to introduce additional amino groups to get amino-gelatin. Upon mixing the A-SA and amino-gelatin aqueous solutions together,a gel rapidly formed based on the Schiff's base reaction between the aldehyde groups in A-SA and the amino groups in amino-gelatin.Fourier transform infrared spectroscopy( FTIR) analysis confirmed the characteristic peak of Schiff's base group in the hydrogel. The gelation time measure has confirmed the gelation time is dependent on the aldehyde group content in A-SA and amino group content in amino-gelatin. The fasted hydrogel formation takes place within 30 s. The entire test suggested that this gel could be a promising candidate as soft tissue adhesive.Sodium alginate and gelatin are both remarkable natural biomaterials; they all have been extensively applied in tissue engineering and other relative fields,due to their low price and good biocompatibility. In this paper,we oxidized sodium alginate with sodium periodate to convert 1,2-hydroxyl groups into aldehyde groups to get aldehyde-sodium alginate（ A-SA）. Gelatin was modified with ethylenediamine（ ED） in the presence of water-soluble1-ethyl-3（ 3-dimethylaminopropyl） carbodiimide（ EDC） to introduce additional amino groups to get amino-gelatin. Upon mixing the A-SA and amino-gelatin aqueous solutions together,a gel rapidly formed based on the Schiff＇s base reaction between the aldehyde groups in A-SA and the amino groups in amino-gelatin.Fourier transform infrared spectroscopy（ FTIR） analysis confirmed the characteristic peak of Schiff＇s base group in the hydrogel. The gelation time measure has confirmed the gelation time is dependent on the aldehyde group content in A-SA and amino group content in amino-gelatin. The fasted hydrogel formation takes place within 30 s. The entire test suggested that this gel could be a promising candidate as soft tissue adhesive.

关 键 词：oxidized sodium alginate amino-gelatin adhesive agent 

分 类 号：TB324[一般工业技术—材料科学与工程] R318.08[医药卫生—生物医学工程]