姜黄素磁性印迹固相萃取材料的制备及其应用研究  

Preparation and Application of Curcumin Magnetic Molecularly Imprinted Solid-phase Extraction Material

作　　者：刘玉楠[1] 张朝晖[1,2] 黄小花[1] 饶维[1] 

机构地区：[1]吉首大学化学化工学院,湖南吉首416000 [2]湖南大学化学生物传感与计量国家重点实验室,湖南长沙410082

出　　处：《林产化学与工业》2014年第6期37-43,共7页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金资助项目(21005030;21267010)

摘　　要：以二氧化硅包覆的四氧化三铁为基材，姜黄素为模板分子，甲基丙烯酸为功能单体，乙二醇二甲基丙烯酸酯为交联剂，采用热聚合技术制备出姜黄素磁性印迹聚合物。采用扫描电镜、差热分析、样品振动磁强计和红外光谱等多种技术对该磁性印迹聚合物的形态、性能和化学结构进行分析和表征，结果表明，在磁性四氧化三铁表面成功制备出姜黄素磁性印迹聚合物。结合高效液相色谱分析技术，对该磁性印迹聚合物的吸附性能进行探讨，该磁性印迹聚合物对姜黄素表现出特异性吸附性能，最大吸附容量计算值为36.2 mg/g；相对于大黄酸和大黄素，姜黄素的选择因子（β）分别是2.0和2.2。结合磁固相萃取技术与液相色谱检测技术，实现了姜粉样品溶液中姜黄素的分离、富集和检测，回收率为99.2%-107%。Magnetic molecularly imprinted polymer（ MIP） was prepared by using magnetic iron oxide as the carrier, curcumin as the template molecule, methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker. The Fe3 O4@ SiO2 @ MIP was characterized by scanning electron microscope, thermogravimetric analysis, vibrating sample magnetometer and infrared spectroscopy. The results showed that the Fe3 O4@SiO2@MIP was prepared on magnetic iron oxide surface successfully. The adsorption properties were investigated by high performance liquid chromatography. The results demonstrated that the Fe3 O4@SiO2@MIP exhibited specific selectivity toward curcumin. The maximum adsorption capacity on Fe3 O4@SiO2@ MIP was 36. 2 mg/g, and the selectivity factors of curcumin toward rhein and emodin were 2. 0 and 2. 2, respectively. Combined with magnetic solid phase extraction and high-performance liquid chromatography technology, the Fe3 O4@SiO2@MIP was used to separate, enrich and detect curcumin in ginger powder, sample solution successfully with the recoveries of 99. 2% -107%.

关 键 词：磁性印迹聚合物 姜黄素 磁固相萃取技术 高效液相色谱技术 

分 类 号：TQ35[化学工程]