杜仲总木脂素及松脂醇二葡萄糖苷的纯化研究  

Purification of Lignans and Pinoresinol Diglucoside from Eucommia ulmoides Oliv.

作　　者：彭密军[1] 吕强[1] 彭胜[1] 张昌伟[1] 张琳杰[1] 王志宏[1] 周云雷[1] 

机构地区：[1]吉首大学林产化工工程湖南省重点实验室,湖南张家界427000

出　　处：《林产化学与工业》2014年第6期88-92,共5页Chemistry and Industry of Forest Products

基　　金："十二五"国家科技支撑计划资助(2011BAI01B08);湖南省教育厅高校产业化培育项目(11CY015);张家界市科技局重点项目(2011ZD03)

摘　　要：通过大孔吸附树脂-C18反相硅胶柱层析-正相硅胶柱层析联用技术,对杜仲总木脂素及松脂醇二葡萄糖苷(PDG)进行纯化研究。结果表明,含总木脂素23.33%和松脂醇二葡萄糖苷粗品0.960 5%的杜仲提取液经AB-8树脂一次纯化,可以制得71.61%的杜仲总木脂素有效部位以及8.070%的松脂醇二葡萄糖苷粗品,再经C18反相硅胶柱层析纯化,得到53.12%的松脂醇二葡萄糖苷。最后通过正相硅胶柱层析进一步纯化,得到91.50%的松脂醇二葡萄糖苷产品。Purification of lignans and pinoresinol diglucoside （ PDG ） from Eucommia bark extract was studied by a combined method of macroporous adsorption resin-C18 column chromatography and silica gel column chromatography. The results indicated that AB-8 resin was the best one on the adsorption and desorption rates by comparing with the other 3 kinds of resins. The purity of effective part of lignans and the crude product of PDG were respectively from 23. 33% to 71. 61% and 0. 960 5% to 8. 070 %through AB-8 resin once. Then the purity of PDG increased to 53. 12 % after C18 purification and finally reached 91. 50 % after purifying by normal phase silica gel column chromatography.

关 键 词：杜仲 总木脂素 松脂醇二葡萄糖苷 纯化工艺 

分 类 号：TQ351[化学工程]