活血止痛凝胶体外释放和经皮渗透性研究  

Study on in vitro release and percutaneous absorption of Huoxue Zhitong gel

作　　者：于娟[1] 杜茂波[2] 刘淑芝[2] 宋立华[1] 沈硕[2] 刘道芳[3] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]中国中医科学院中药研究所,北京100700 [3]安徽安科余良卿药业有限公司,安徽安庆246001

出　　处：《中国中药杂志》2014年第24期4778-4781,共4页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2014ZX09301306-009)

摘　　要：采用Franz扩散池进行实验,以半透膜和小鼠皮肤为透过屏障,采用HPLC测定接受液[PEG400-95%乙醇-水(1∶3∶6)]中丹皮酚的含量,考察活血止痛凝胶体外释放和经皮渗透规律。结果显示,活血止痛凝胶中丹皮酚的释药规律符合Weibull方程,拟合方程为ln[-1/(1-Q)]=-0.790 5lnt-1.701 2(r=0.980 9),在8 h内丹皮酚的累积释放率为85.18%,释放速率为2.827μg·cm-2·h-1;丹皮酚的累积经皮渗透率以零级模型拟合好,拟合方程为Q(t)=1.757 9t+0.721 3(r=0.999 1),给药8 h后丹皮酚的累积经皮渗透率为54.85%,渗透速率为1.820μg·cm-2·h-1。研究结果表明,活血止痛凝胶具有良好的释放透皮性能。To evaluate in vitro release and transdermal behaviors of Huoxue Zhitong gel, modified Franz diffusion cell methods was applied to investigate in vitro transdermal absorption of Huoxue Zhitong gel and the content of paeonolan in receptor fluid composed of PEG400%-95% ethanol-water（ 1： 3： 6）were determined by HPLC. The results were processed and different equations were fitted. The release law were in accordance with Weibull equation and the fitting equation was ln[ - 1/（ 1 - Q） ] = -0. 790 51nt - 1. 701 2 （r =0. 980 9）. In 8 hours, cumulative release of paeonol was 85.18% and the release rate was 2. 827 μg ·cm^-2 · h^-1. Transdermal actions were consistent with zero-level model fit and the fitting equation was Q（t） = 1. 757 9t ＋ 0. 721 3 （ r = 0. 999 1 ）. In 8 hours, cumulative transdermal rate and transmission rate of paeonol was 54. 85%, 1. 820 μg·cm^-2·h^-1. So the Huoxue Zhitong gel had a good release and transdermal properties.

关 键 词：释放 透皮 丹皮酚 HPLC 活血止痛凝胶 

分 类 号：R285.5[医药卫生—中药学]