MS2病毒样颗粒原核表达优化及颗粒稳定性能测试  

Expressional Optimization of Prokaryotic Expression Vector of MS2 Phage and Assay of the Stability of Virus-like Particles

作　　者：张国广[1,2] 欧阳莹[2] 刘颖[2] 黄小君[2] 陈亮[2] 

机构地区：[1]闽南师范大学生物科学与技术学院,福建漳州363000 [2]厦门大学生命科学学院,福建厦门361005

出　　处：《闽南师范大学学报（自然科学版）》2014年第4期62-66,共5页Journal of Minnan Normal University：Natural Science

摘　　要：MS2噬菌体病毒样颗粒广泛用于构建RNA病毒检测中的阳性对照品.本文基于已构建的表达载体p CPHA,优化了表达载体在BL21(DE3)细胞中的表达条件.结果表明在LB培养基中,菌液OD600=0.9时,加入IPTG诱导6h,外壳蛋白的表达量达到最大,电镜下观察到表达出的产物呈现MS2噬菌体病毒颗粒结构,该病毒样颗粒在常温下至少可以保存60 d,64℃温度时30 min内结构保持稳定.Virus-like particle of MS2 bacteriophage was widely used as a kind of positive control in detection of RNA virus. Several expressional conditions of pCPHA vector that pack hemagglutinin （HA） gene segment of avian influenza virus were optimized inside E. eoli BL21 （I）E3） eel. The results showed coat protein expression level reaehes the maximum at the broth OD_600 of 0.9, IPTG induction 6h and LB liquid medium. Expression product morphological structure was the same as MS2 bacteriophage virus particles under scanning electron microscope. The structure of virus-like particle was stable at normal temperature for at least 60d and 64℃ for at least 30min.

关 键 词：MS2噬菌体 病毒样颗粒 表达量优化 稳定性能 

分 类 号：Q786[生物学—分子生物学]