CIMMYT小麦Kingbird和PBW343中条锈病和叶锈病成株抗性QTL分析  

QTL Mapping of Adult-plant Resistance Genes to Leaf Rust and Stripe Rust in CIMMYT Wheat Kingbird and PBW343

作　　者：张培培[1] 张亚琦[1] 李寒冰[1] 高璞[1] 李柳[1] 李星[1] 李在峰[1] 刘大群[1] 

机构地区：[1]河北农业大学植物保护学院/河北省农作物病虫害生物防治工程技术研究中心,河北保定071001

出　　处：《河南农业科学》2014年第11期82-86,96,共6页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31361140367);河北省自然科学基金项目(C2014204113);河北省教育厅优秀青年项目(YQ2013024)

摘　　要：为检测和定位CIMMYT小麦Kingbird和PBW343中的成株慢锈QTL,以Kingbird和PBW343作为亲本进行杂交、多次自交得到的181个F6代重组自交系(RILs)为材料,种植于墨西哥田间进行抗叶锈病和条锈病鉴定,获得群体的表现型数据。同时利用406个在亲本间有多态性的多样性序列芯片技术(DArT)标记检测181个家系,获得群体的基因型数据。结合表现型数据和基因型数据,利用软件Map manager QTXb20和QTL IciMapping 3.2软件,采用复合区间作图法进行成株抗叶锈和条锈QTL分析,结果共检测到3个抗叶锈QTL和1个抗条锈QTL,分别位于1A、7DS、5BL、3BS染色体上,解释5.57%、11.94%、5.11%、9.03%的表型变异。其中,位于7DS和3BS染色体上的QTL来自Kingbird,位于1A和5BL染色体上的QTL来自PBW343,这些成株慢锈QTL丰富了现有的小麦成株慢锈基因库。In order to detect and map the QTL to leaf rust and stripe rust in CIMMYT wheat lines Kingbird and PBW343,a total of 181 recombinant inbred lines （RILs）derived from the cross Kingbird and PBW343 were planted in Mexico to score disease severity of leaf rust and stripe rust to obtain the phenotypic data.A total of 406 DArT markers with polymorphism between parents were used to test the whole 1 8 1 RILs to obtain the genotypic data.Phenotypic data and genotypic data were used to map QTL by softwares Map manager QTXb20 and QTL IciMapping 3.2 with composite interval mapping strategy.The results showed that a total of three QTLs for leaf rust resistance and one QTL for stripe rust resistance were detected on chromosome 1A,7DS,5BL and 3BS,respectively,and explaining 5.57%,11.94%,5.11% and 9.03% of the phenotypic variance. The QTLs on 7DS and 3BS were from Kingbird,and the other two QTLs were detected in PBW343.These QTLs enrich the wheat slow rusting gene pool and will give theory and technology support for wheat resistance breeding.

关 键 词：小麦 叶锈病 条锈病 成株慢锈基因 QTL作图 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]