金花茶CnCHS基因原核表达载体的构建及表达分析  

Construction of Prokaryotic Expression Vector and Expression of Chalcone Synthase Gene from Camellia nitidissima

作　　者：周兴文[1] 李纪元[2] 朱宇林[1] 孙迎坤[3] 

机构地区：[1]玉林师范学院生命科学与技术学院,广西玉林537000 [2]中国林业科学研究院亚热带林业研究所,浙江富阳311400 [3]青岛农业大学园林与林学院,山东青岛266109

出　　处：《河南农业科学》2014年第11期104-106,共3页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31360197);广西自然科学基金项目(2012GXNSFBA053077);广西高校科研一般项目(201203YB155);玉林师范学院高层次人才科研启动基金(G20120027)

摘　　要：根据已经克隆得到的金花茶(Camellia nitidissima Chi.)查尔酮合成酶基因序列,设计引物对P1/P2扩增出该基因全长并将其构建到原核表达载体pGEX-4T-1中,转入大肠杆菌BL21后,用浓度为0.4mmol/L的IPTG诱导其表达。SDS-PAGE电泳检测结果显示,被诱导的大肠杆菌菌体中出现了约68ku的特异融合蛋白,表明该基因在大肠杆菌中成功表达。Full length gene fragment of chalcone synthase from Camellia nitidissima was cloned by primers P1 and P2 which were designed according to the sequence of CnCHS.The CnCHS gene was connected to prokaryotic expression vector pGEX 4T 1 and induced by 0.4 mmol/L IPTG after the recombinant plasmid was transferred to Escherichia coli BL2 1 .SDS PAGE results showed that a special band with molecular weight of 68 ku appeared,indicating that the CnCHS gene was expressed successfully in E.coli BL2 1 .

关 键 词：金花茶 查尔酮合成酶 原核表达 

分 类 号：S685.14[农业科学—观赏园艺]