亚硒酸钠对人增生性瘢痕成纤维细胞体外增殖的影响  

Effect of sodium-selenite on human hypertrophic scar fibroblasts proliferation in vitro

作　　者：杨陆涛[1] 刘美玲[1] 张友来[1] 辛国华[1] 李国辉[1] 曾元临[1] 

机构地区：[1]南昌大学第一附属医院烧伤中心,南昌330006

出　　处：《重庆医学》2014年第35期4723-4726,共4页Chongqing medicine

摘　　要：目的观察亚硒酸钠对人增生性瘢痕成纤维细胞体外增殖的影响。方法体外培养人增生性瘢痕成纤维细胞,取第4代细胞用CCK-8检测成纤维细胞的增殖情况,分为试验组和对照组,试验组分为6组（A、B、C、D、E、F组）,分别加入等量含2.5、5.0、10.0、20.0、40.0、80.0μmol/L浓度亚硒酸钠的10%胎牛血清培养基;对照组加入等量的10%胎牛血清培养基,分别在24、48、72、96h用CCK-8试剂盒检测细胞增殖情况;Live/dead试剂检测加入不同浓度亚硒酸钠24h后细胞的存活情况;细胞免疫组织化学法检测加入不同浓度亚硒酸钠24h后,细胞内Ⅰ、Ⅲ型胶原的表达情况。结果 （1）亚硒酸钠在2.5-80.0μmol/L浓度范围内,在同一时间内随着浓度的增加,对成纤维细胞的抑制率逐步增加（P〈0.05）;（2）在相同浓度作用下随着时间的延长,亚硒酸钠对成纤维细胞的抑制率逐渐增加（P〈0.05）;（3）Live/dead试剂检测结果显示,随着亚硒酸钠浓度的增加,凋亡细胞数逐渐增加;（4）随着亚硒酸钠的浓度增加,成纤维细胞内Ⅰ、Ⅲ型胶原表达逐渐减弱。结论亚硒酸钠在体外能抑制人增生性瘢痕成纤维细胞的增殖,并且可以降低成纤维细胞内Ⅰ、Ⅲ型胶原的表达。Objective To observe the effects of sodium-selenite on human hypertrophic scar fibroblasts proliferation in vitro.Methods Human hypertrophic scar fibroblast culture was conducted in vitro,the status of fibroblast proliferation of the 4th generation cells was tested by CCK-8,which was divided into experimental group and control group,the experimental group was divided into six groups（A,B,C,D,E,F）,and were added an equal volume-containing 2.5,5.0,10.0,20.0,40.0,80.0μmol/L concentrations of sodium selenite in 10% FBS culture medium;the control group added an equal volume of 10% FBS culture medium,testing cell proliferation by CCK-8at 24,48,72,96hrespectively;testing different concentrations of sodium-selenite cell survival situation after 24 hby Live/dead reagent;immunohistochemical was used to test intracellularⅠ,Ⅲ type collagen expression after 24 h.Results（1）With the increased of concentration,the inhibition rate of fibroblasts gradually increased as the concentration of sodiumselenite ranged in 2.5-80.0μmol/L（P〈0.05）;（2）the inhibition rate of sodium-selenite on fibroblasts gradually increased at the same concentration with time（P〈0.05）;（3）Live/dead reagent test results showed that apoptosis cell number increased with the concentration increasing;（4）With concentrations of sodium-selenite increasing,typeⅠ,Ⅲcollagen expression of fibroblast decreased gradually.Conclusion Sodium-selenite can inhibit human hypertrophic scar fibroblast proliferate in vitro and reduceⅠ,Ⅲcollagen expression of fibroblast type

关 键 词：亚硒酸钠 瘢痕 成纤维细胞 增殖 

分 类 号：R622[医药卫生—整形外科]