成人外周血CIK细胞异常升高病例临床实验资料分析  

A clinic laboratory data analysis of peripheral CIK cell abnormal increase in adult

作　　者：但刚[1] 毛怀凤 吴丽娟[1] 刘毓刚[1] 刘彤[1] 谢静[1] 胡晓莉[1] 王艳艳[1] 

机构地区：[1]成都军区总医院临床实验医学研究与保障中心,成都610083 [2]四川省阿坝州人民医院检验科,624000

出　　处：《重庆医学》2014年第35期4727-4730,共4页Chongqing medicine

基　　金：成都军区总医院创新课题资助(42412EIA)

摘　　要：目的分析成人外周血CIK细胞异常升高病例的临床实验资料,探讨实验诊断指标在该疾病诊断、病情监测与治疗干预中的作用。方法采集患者外周血进行细胞分析,流式细胞仪检测淋巴细胞亚群,对患者进行生物化学分析、凝血功能检测和骨髓细胞学检查,并对该患者进行病案回顾性分析。结果患者外周血白细胞总数和淋巴细胞百分含量升高;免疫分型结果显示,淋巴细胞主要是T细胞,T8细胞升高,T4细胞减少;细胞中CIK细胞（CD3＋CD56＋）占绝大多数。治疗后,CIK细胞含量稍有下降,在28.6%-59.7%波动。治疗前、后骨髓细胞学检查无明显异常。外周血TNF-α及IgM轻度升高,补体C4轻度下降,其余各项指标均无明显异常。结论发现了成人外周血CIK细胞特发性升高病例,流式T淋巴细胞亚群监测可为临床提供诊断、疗效监测信息,值得推广。Objective To analyze the clinic data of peripheral CIK cell increased abnormally in adults and discuss the function of the laboratory indexes on disease diagnosis,monitoring and therapeutic intervention.Methods The peripheral blood of patients was collected to analysis.The flow cytometry instrument was used to detect lymphocyte subgroup.every patient was conducted biochemical analysis,function of blood coagulation tests and examination of marrow cell and the patient′s medical record was analyzed retrospectively.Results The total number of leukocyte and the percentage of lymphocyte in peripheral blood of patient were increased;immunophenotyping results showed that lymphocytes was mainly T lymphocyte,T8 cell number was increased and number of T4 cell was decreased;CIK（CD3＋56＋）cells were in the majority.the number of CIK cells was decreased after treatment and ranged from 28.6%to 59.7%.there was no obvious anomaly in examination of marrow cell before and after treatment.TNF-α,IgM were increased mildly,C4 was decreased mildly,and other indicators were no obviously abnormal.Conclusion It is the first time that cases with CIK cells idiopathic increased.The monitoring of flow T lymphocyte subgroup can provide clinical diagnosis,effect monitoring and it is worth to promote.

关 键 词：T淋巴细胞亚群 细胞因子诱导的杀伤细胞 流式细胞术 

分 类 号：R446.1[医药卫生—诊断学]