温郁金醚提物中二萜类化合物C体外对人结肠腺癌细胞SW620的抑制作用  

Inhibitory effects of diterpenoid compound C abstracted from Radix Curcumae ether on human carcinoma of colon cells SW620 in vitro

作　　者：沈雁[1] 吕宾[2] 张烁[2] 马忠俊[3] 李思[4] 

机构地区：[1]浙江中医药大学附属第二医院消化内科,杭州310005 [2]浙江中医药大学附属第一医院消化内科,杭州310006 [3]浙江大学药学院现代中药研究所,杭州310058 [4]浙江省立同德医院儿科,杭州310012

出　　处：《中华中医药杂志》2014年第12期3801-3805,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省中医药普通课题研究计划(No.2009CB014)~~

摘　　要：目的:探讨温郁金醚提物中二萜类化合物C体外对人结肠腺癌SW620细胞的抑制作用及机制。方法:将不同浓度的化合物C作用SW620细胞后,采用MTT法检测细胞的增殖情况,倒置相差显微镜下观察细胞的形态学变化,流式细胞术检测细胞的周期分布和凋亡率。结果:化合物C能以时间-浓度依赖性方式抑制SW620细胞增殖,其抑制能力显著高于阳性对照药5-Fu和L-OHP(P<0.01),而对正常HUVEC细胞仅有轻微毒性作用;作用后的细胞在镜下呈典型的凋亡改变;化合物C能阻滞细胞于G1和S期,减少G2期比例,并以时间-浓度依赖性方式显著诱导细胞凋亡(P<0.01)。结论:温郁金中二萜类化合物C能显著抑制SW620细胞的增殖,并阻滞细胞周期和诱导细胞凋亡,这可能是其发挥抗肿瘤作用的机制之一。Objective： To investigate the inhibitory effects of diterpenoid compound C abstracted from Radix Curcumae ether on human carcinoma of colon cells SW620 in vitro and its underlying mechanism. Methods： After treatment with diterpenoid compound C of different concentrations, the growth and proliferation of SW620 cells were reflected by MTT, the morphology of SW620 cells were observed by the inverted phase contrast microscope, and the cycle distribution and apoptosis were detected by flow cytometry. Results： The proliferation of SW620 cells were inhibited by diterpenoid compound C in a time and dose- depending manner, and the inhibitory effect of diterpenoid compound C was significantly higher than that of 5-Fu and L-OHP （P〈0.01）. And there were some mail adverse reactions on non-tumorigenic HUVEC cells. After treatment with diterpenoid compound C of different concentrations, the apoptosis change of SW620 cells were observed under the inverted phase contrast microscope. Diterpenoid compound C could inhibit the proliferation of SW620 cells at G1 and S phases and reduce the proportion at G2 phase, and diterpenoid compound C could markedly induce the apoptosis in a time and dose-depending manner （P〈0.01）. Conclusions： The diterpenoid compound C abstracted from Radix Curcumae ether can significantly inhibit the proliferation, block cycle and induce apoptosis of human carcinoma of colon cells SW620 in vitro, which may be one of the mechanisms of its antitumor effect.

关 键 词：结肠腺癌 温郁金 细胞增殖 细胞凋亡 

分 类 号：R285[医药卫生—中药学]