解毒三根汤对结肠癌相关成纤维细胞VEGF、IL-6的影响及对Caspase信号通路的探讨  

Research on effect of Jiedu Sangen Decoction on the expression of VEGF, IL-6 and Caspase signal pathway in colon cancer associated fibroblasts

作　　者：阮善明[1,2] 沈敏鹤[1] 郑丽萍[2] 林红[2] 张恺[2] 

机构地区：[1]浙江中医药大学附属第一医院肿瘤科,杭州310006 [2]浙江中医药大学第一临床医学院,杭州310053

出　　处：《中华中医药杂志》2014年第12期3936-3939,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81373632);国家公益性行业科研专项项目(No.200807018);浙江省教育厅项目(No.Y201016272);浙江省中医药优秀青年人才基金计划(No.2013ZQ011)~~

摘　　要：目的:探讨解毒三根汤(虎杖根、水杨梅根、藤梨根)对肿瘤相关成纤维细胞(CAFs)的血管内皮生长因子(VEGF)、白介素-6(IL-6)的影响以及对Caspase信号通路的探讨。方法:BALB/c小鼠皮下接种CT-26结肠癌细胞(1.2×106m L);随机分为3组:正常对照组、模型组、解毒三根汤组,ELISA检测3组CAFs上清液中VEGF、IL-6的表达量,实时荧光定量PCR检测各组CAFs中Caspase-3、Caspase-8的m RNA表达量。结果:24h及48h解毒三根汤组刺激的CAFs上清液中VEGF、IL-6的表达量较模型组显著降低(P<0.01);解毒三根汤组Caspase-3、Caspase-8 m RNA表达量较模型组明显上升(P<0.01)。结论:解毒三根汤降低CAFs上清液中VEGF、IL-6的表达量,提高CAFs中Caspase-3、Caspase-8 m RNA的表达量,可能是其抑制肿瘤细胞迁移侵袭的机制。Objective： To observe the effect of Jiedu Sangen Decoction （JSD, consisting of Polygonum cuspidatum, Geum japonicum thumnb, Radix actinidiae） on the expression of carcinoma-associated fibroblasts （CAFs） such as vascular endothelial growth factor （VEGF）, IL-6 and caspase signal pathway. Methods： The BALB/c mice were subcutaneously inoculated with colon cancer CT-26 cells （1.2×10^6mL） and then randomly divided into 3 groups, a normal control group, a model group, a JSD group. The expression of VEGF and IL-6 in the supernatant of CAFs was detected by using ELISA. The mRNA expression of caspase-3 and caspase-8 was detected by real-time fluorescence quantitative PCR. Results： Compared with the model group, the expression of VEGF and IL-6 in the supernatant of CAFs decreased in the JSD group at 24h and 48h （P〈0.05, P〈0.01）. The mRNA expression of caspase-3 and caspase-8 in the JSD group increased obviously compared with the model group, and the difference was statistically significant （P〈0.01）. Conclusion： JSD could decrease the expression of VEGF and IL-6 in the supernatant of CAFs, and increase the mRNA expression of caspase-3 and caspase-8. This might be possible mechanisms for inhibiting the migration and invasion of tumor cells.

关 键 词：虎杖根 水杨梅根 藤梨根 肿瘤相关成纤维细胞 血管内皮生长因子 白介素-6 Caspase-3 CASPASE-8 

分 类 号：R285[医药卫生—中药学]