固本止咳中药对COPD小鼠肺组织γδT细胞及IL-17的影响  

Effects of Gubenzhike recipe on gamma delta-T cell and IL-17 in lung of COPD mice

作　　者：刘颖[1] 敬岳[1] 郭丽丽[2] 舒峻[2] 秦玉英[1] 谷明杰 潘琳[2] 李鸿[2] 马秉智[2] 蔡哲[2] 张洪春[2] 

机构地区：[1]北京中医药大学研究生院,北京100029 [2]中日友好医院,北京100029

出　　处：《中华中医药杂志》2014年第12期3981-3984,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81072767)~~

摘　　要：目的:观察固本止咳中药对COPD模型小鼠肺组织上皮细胞间γδT细胞及IL-17影响,探讨其治疗COPD的作用机制。方法:采用鼻腔滴入LPS加熏香烟的方法建立COPD小鼠模型,实验分为空白对照组、模型对照组、固本止咳中药组;运用HE染色对形态学进行观察,评价COPD小鼠模型;应用流式细胞术检测肺组织上皮间γδT淋巴细胞表达情况;并应用ELISA方法检测小鼠肺组织上皮细胞间淋巴细胞培养上清液IL-17分泌水平。结果:COPD小鼠模型形态学结果符合COPD临床病理形态学改变;模型对照组较空白对照组γδT淋巴细胞比例明显升高(P<0.05);中药治疗组较模型对照组γδT淋巴细胞比例明显降低(P<0.05)。淋巴细胞培养上清液中IL-17含量与γδT细胞变化趋势同步。结论:固本止咳中药通过调节γδT细胞的生物活性,减少IL-17的分泌,从而减轻气道炎症,减少气道壁损伤-修复过程的发生,改善了呼吸道黏膜免疫功能。Objective： To observe the effects and mechanism of Gubenzhike recipe on epithelial gamma delta-T cell in lung and IL-17 of COPD mice. Methods： The mouse COPD model was established by exposure to cigarette smoke in combination with intra- nostrils injection of lipopolysaccharide. The mice were divided into three groups： negative control group, model group and Gubenzhike recipe group; Use the methods of HE eosin staining to observe the morphology characteristic of COPD model; Use the method of Flow cytometry to test the expression of epithelial γδ T cell in lung. Use the ELISA to test the IL-17 level in supernatant of epithelial lymphocyte culture, Results： The morphology characteristic was Up to COPD; The proportion of γδT cells of model group was highel： than negative control group （P〈0.05）; And the proportion of γδT cells of Gubenzhike recipe group was lower （P〈0.05）; The IL-17 level in supernatant of epithelial lymphocyte culture coincided with the changing trend of γδT cell.Conclusion： Gubenzhike recipe might improve respiratory mucosal immune function by regulating the biological activity of γδT cells, which reduce the secretion of IL-17, relieve airway inflammation and reduce damage and repair process of the airway.

关 键 词：慢性阻塞性肺疾病 固本止咳中药 黏膜免疫 ΓΔT细胞 IL-17 

分 类 号：R285.5[医药卫生—中药学]