基于抑制消减杂交技术筛选AM真菌与紫穗槐共生相关基因  

Screening the Related Genes in the AM Fungi and Amorpha fruticosa Symbiosis with the Suppression Subtractive Hybridization Technique

作　　者：宋福强[1] 孔祥仕[1] 李季泽[1] 常伟[1] 

机构地区：[1]黑龙江大学生命科学学院,哈尔滨150080

出　　处：《林业科学》2014年第11期64-74,共11页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(31070576;30571493);黑龙江省自然科学基金重点项目(ZD201206);黑龙江省杰出青年科学基金项目(JC201306);黑龙江省大学高层次人才支持计划项目(Hdtd2010-12)

摘　　要：利用抑制消减杂交技术筛选AM真菌在侵染紫穗槐的过程中紫穗槐差异表达的基因,共获得47个unigenes,其中35个显著匹配。根据GO分类信息、Blast注释信息和相关文献信息,显著匹配的35个unigenes基因按功能分为防御胁迫(24%)、物质代谢(17%)、能量代谢(14%)、蛋白合成(11%)、信号转导(11%)、细胞结构构建(11%)、转录(6%)、蛋白折叠和降解(6%)8类。其中,胁迫防御相关基因和代谢相关基因所占比例最大,表明二者在AM真菌和紫穗槐共生互作过程中起重要作用。这些基因广泛参与生物体生命活动的各个过程,因此AM真菌与紫穗槐共生的过程是一个系统涉及各种生物过程的活动。In this study, the suppression subtractive hybridization （ SSH ） method was employed to screen the differential expressed genes in Amorpha fruticosa roots induced by AMF during the colonization process. The results showed that 47 unigenes were obtained,and 35 of them could be aligned with known function genes in GenBank through Blast. According to GO classified information,Blast annotation and related literatures,the 35 unigenes were assigned into eight categories including stress and defense （24%） ,substance metabolism （17%） ,energy metabolism （14%） , protein synthesis （ 11%） ,signaling （ 11%） ,cellular structre/ cytoskeleton （ 11%） ,transcription related （ 6%） , protein folding and degradation related （6%） . Among them,stress and defense genes,and metabolism genes occupied the largest fraction,implying stress and defense related genes and substance metabolism related genes should play an important role in the symbiosis process between A. fruticosa and AMF. These genes are widely involved in the biological process of life activity,thus,the process of AMF and A. fruticosa symbiosis is a system involving a variety of biological activities.

关 键 词：紫穗槐 丛枝菌根真菌 抑制消减杂交技术 共生相关基因 

分 类 号：S718.81[农业科学—林学]