广东淇澳岛3种红树rDNA ITS序列分析  

Analysis of rDNA ITS Sequence of Three Mangrove Species in Qi'ao Island,Guangdong

作　　者：彭莉萍[1] 牛宪立[1] 姬可平[1] 王燕[1] 

机构地区：[1]遵义医学院珠海校区,广东珠海519041

出　　处：《贵州农业科学》2014年第11期29-32,共4页Guizhou Agricultural Sciences

摘　　要：为红树林植物引进优良种质资源,用改良CTAB法分别提取生长于广东珠海淇澳岛红树林湿地公园的3种红树基因组DNA,以真核生物rDNA ITS序列的通用引物分别对其ITS区进行PCR扩增后测序,对测序结果进行比对、分析。结果表明,不同来源红树的rDNA ITS序列存在扩增碱基差异,无瓣海桑、尖叶卤蕨和猫尾木的rDNA ITS序列长度分别为731bp、615bp和707bp,将3种红树植物ITS序列测定结果提交到GenBank,获得了登录号分别为KJ161168,KJ161166,KJ161167。The genome DNA of three mangrove species from Qi＇ao island,Guangdong was extracted by the modified CTAB method,then the rDNA ITS amplified by PCR with eukaryotic Ponsensus primers was sequenced,and finally the sequencing results were compared and analyzed to provide a reference for introduction of excellent mangrove germplasm resources.There is difference in the amplified base of rDNA ITS sequence among different mangrove species.The rDNA ITS sequence length of Sonneratia apetala,A.speciosumand D.caudafelinais 731 bp,615bp and 707 bp respectively.The accession number of ITS sequence of S.apetala,A.speciosum and D.caudafelina is KJ161168,KJ161166 and KJ161167in GenBank separately.

关 键 词：红树 PCR ITS序列 

分 类 号：S727.5[农业科学—林木遗传育种]