国庆菊继代增殖培养技术初探  

Subculture Multiplication Technique of Chrysanthemum morifolium

作　　者：袁丽伟[1] 

机构地区：[1]河北旅游职业学院,河北承德067000

出　　处：《贵州农业科学》2014年第11期36-38,共3页Guizhou Agricultural Sciences

基　　金：河北省承德市科技计划项目"承德地区野生观赏植物资源调查及引种栽培"阶段研究成果(20132218)

摘　　要：为国庆菊的工厂化快繁提供理论依据,选取初代培养完成的国庆菊截取茎段作为外植体,分别以MS、N6和White为基本成分,附加不同浓度细胞分裂素(6-BA)组成12种继代增殖培养基,研究了国庆菊继代增殖培养技术。结果表明:不同培养基培养,第11天开始长新叶,第15天开始长丛生芽,30d后增殖情况差异明显,MS培养基的丛生芽最多,且旺盛;N6培养基的丛生芽较MS少但比White多且旺盛,White培养基的丛生芽最少且最弱。细胞分裂素浓度为1.5mg/L的MS培养基的国庆菊丛生芽增殖倍数最大,达17.5;其次是浓度为0.5mg/L的N6培养基,为11.5;浓度为0.5mg/L的White培养基的丛生芽增殖倍数最小,仅1.7。MS是继代增殖培养的最适培养基,1.5mg/L是最适细胞分裂素浓度。Stem segments of Chrysanthemum morifolium after primary culture were cultured on MS,N6,and White media plus 6-BA with different concentration respectively to study the subculture propagation technique of C.morifolium and to provide the theoretical basis for industrialization rapid propagation of C.morifolium.Stem segments cultured on different media start to grow young leaves after11 d,grow cluster buds after 15 dand there are obvious differences in multiplication between different media after 30 d.The number and growth vigor of cluster buds cultured on MS medium〉N6 medium 〉White medium.The multiplication coefficients of cluster buds cultured on MS with 1.5mg/L 6-BA,N6 with 0.5mg/L 6-BA and White with 0.5mg/L 6-BA are 17.5,11.5and 1.7separately.The optimum medium and6-BA concentration for subculture propagation of C.morifoliumare MS medium and 1.5mg/L.

关 键 词：国庆菊 培养基 细胞分裂素 继代培养 

分 类 号：S682.11[农业科学—观赏园艺]