龙胆属5种植物的同工酶比较  

Study on Isoenzymes of Five Species in Gentiana

作　　者：孙爱群[1] 游进国[1] 林长松[1] 李鹏[1] 

机构地区：[1]六盘水师范学院生命科学系,贵州六盘水553004

出　　处：《贵州农业科学》2014年第11期62-65,共4页Guizhou Agricultural Sciences

基　　金：贵州中药现代化项目"贵州龙胆属药用植物资源调查及利用评价研究"(黔科合ZY字20123007);六盘水市科学技术局项目"民族药红花龙胆的驯化栽培及快繁技术研究"(52020-2012-01-09-06);六盘水市科学技术局创新人才团队建设计划项目(52020-2012-04-01-01)

摘　　要：为探明龙胆属植物种间亲缘关系,采用聚丙烯酰胺垂直凝胶电泳技术,对龙胆属红花龙胆Gentiana rhodantha、草甸龙胆G.praticola、翼萼龙胆G.pterocalyx、滇龙胆G.rigescens和头花龙胆G.cephalantha的过氧化物酶同工酶(POD)、超氧化物歧化酶同工酶(SOD)和淀粉酶同工酶(AMY)的酶谱和亲缘关系进行比较。结果显示:1)5种龙胆属植物的3种同工酶共显示29条酶带,其酶带数和相对迁移率不同,酶活性也存在差异;2)红花龙胆与翼萼龙胆的亲缘关系较近,滇龙胆与头花龙胆的亲缘关系也较近。5种龙胆的聚类分析结果与形态分类结果一致。The POD,SOD and AMY zymogram and genetic relationship of G.rhodantha,G.praticola,G.pterocalyx,G.rigescens and G.cephalantha were analyzed and compared by polyacrylamide vertical gel electrophoresis to study the genetic relationship among five different species in Gentiana.The results showed that：1）there are 29 enzyme bands in POD,SOD and AMY isozymes of five species in Gentiana,and there are differences in their enzymatic activity because of different enzyme bands and relative mobility;2）The genetic relationship between G.rhodantha and G.pterocalyxis close,and the genetic relationship between G.rigescens and G.cephalantha is close also.The clustering analysis of five species in Gentianais not different with their morphological classification results.

关 键 词：龙胆属 过氧化物酶同工酶 超氧化物歧化酶同工酶 淀粉酶同工酶 聚类分析 

分 类 号：S567[农业科学—中草药栽培]