尘螨变应原Der f 9、Der p 9和Blo t 9结构与功能的比较研究  

Structural and Functional Comparison of the Dust Mite Allergen Der f 9, Der p 9 and Blo t 9

作　　者：滕飞翔[1] 孙金霞[1] 俞黎黎[1] 卞勇华[1] 王运刚[1] 王楠[1] 张承伯 崔玉宝[1] 

机构地区：[1]盐城卫生职业技术学院医学检验学院,中国江苏盐城224005

出　　处：《生命科学研究》2014年第6期483-487,共5页Life Science Research

基　　金：国家自然科学基金面上项目(NSFC31272369)

摘　　要：使用多种生物信息学工具来预测、比较尘螨变应原Der f 9、Der p 9和Blo t 9的一级、二级、三级结构及抗原表位,找出3种蛋白结构及功能的异同。Der f 9、Der p 9和Blo t 9氨基酸序列一致性为82.07%,都含有3个胰蛋白酶活性位点氨基酸及2个功能结构区特异性序列;二级和三级结构中都由α-螺旋、β-折叠和无规卷曲组成;活性位点氨基酸在三级结构中完全重合,并相互靠近构成蛋白酶的活性中心;主要潜在抗原表位区域都为5个,并在第47-49aa和200-203aa区域出现了重合,但表位重合区的氨基酸种类不完全相同。应用生物信息方法预测和比较了Der f 9、Der p 9和Blo t 9结构与抗原方面的信息,为进一步研究变应原第9组分的生物学功能、疫苗研制奠定了基础。The physiochemical characterization, sequence alignment, secondary structure, three dimensional （3D） structure and epitopes of the dust mite allergen Der f 9, Der p 9 and Blot 9 were analyzed by bioinformatics methods. According to results of alignment, Der f 9, Der p 9 and Blot 9 shared 82.07% sequence identity. Der f 9, Der p 9 and Blot 9 all contain three active sites and two trypsin functional domains, which showed high identity of amino acid residues. The active sites at the centers of Der f 9, Der p 9 and Blot 9 are closely matched in the overlay of 3D structures. Secondary and 3D structure of three proteins all con- tains a-helices, r-sheets and random coils. Epitopes analysis revealed that Der f 9, Der p 9 and Blo t 9 all have 5 main potential epitopes located in random coils. Epitope sequences of Der f 9, Der p 9 and Blot 9 overlap in three domains （peptides of 47-49aa and 200-203aa）, but the residues in these three domains are not identical. Our studies lay the foundation for biochemical and genetic analysis of these 3 allergens, and may contribute to vaccine development for allergen-specific immunotherapy.

关 键 词：尘螨 变应原 结构 抗原表位 

分 类 号：Q71[生物学—分子生物学]