CMC-膨润土固定高效菌生物降解萘的过程  

The Process of Biodegrading Naphthalene by Fixed Efficient Bacteria with CMC-bentonite

作　　者：高晋云[1] 段炼[1] 孙玥[1] 王红[1] 谢鲜梅[1] 

机构地区：[1]太原理工大学化学化工学院,山西太原030024

出　　处：《非金属矿》2014年第6期58-61,共4页Non-Metallic Mines

基　　金：国家自然科学基金青年基金(201301126);山西省青年科技研究基金(2013021009-3);山西省科技发展计划工业攻关项目(20130321033-02);太原市科技项目(120238)

摘　　要：采用包埋法,以CMC-膨润土混合凝胶为载体,将从活性污泥中驯化分离出的高效菌株固定,制备固定化颗粒,应用于吸附降解萘的过程。研究结果表明,固定化颗粒吸附降解萘的机制包括4个过程,即:吸附-脱附-再吸附-生物降解,同时这4个过程与固定化颗粒内部的细菌生长繁殖呈现对应关系。以6%的接种量(V/V水溶胶)且未经去营养化处理制备得到的固定化颗粒降解萘的效果最佳,在30℃下,32 h内将初始质量浓度为40 mg/L的萘液降解至检测不到的水平。对固定化颗粒进行反复实验,结果显示该固定化颗粒不仅机械强度较好,在130 r/min的条件下连续运行20 d不破碎,而且在反复实验中的耐受性也大大增强,一直保持较高的生物活性,萘的降解率维持在100%,基本不变。A strain was isolated from the activated sludge to degrade naphthalene effectively. Bentonite and carboxyl-methyl cellulose（CMC） were selected as the carriers to immobilize the strain. The process of the immobilized beads degrading naphthalene was investigated. The results showed that the immobilized beads degrading naphthalene consisted of four steps： adsorption- desorption- re-adsorption- biodegradation, and there was a corresponding relation between the four steps and the growth of bacteria in the immobilized beads. The optimum experimental conditions were of 6%（V / Vaqueous sol） suspension without the nutritional treatment, 40 mg/L of the initial naphthalene concentration and 30 ℃. Naphthalene was degraded to the undetectable level in 32 h. The reuse of the immobilized beads allows us to get 10 cycles. During the reusing process, the tolerance and biological activity of the immobilized beads were greatly enhanced, and the naphthalene degradation rate was almost remained unchanged at 100%.

关 键 词：萘 生物降解 固定化颗粒 CMC-膨润土 

分 类 号：X703[环境科学与工程—环境工程] TD985[矿业工程—选矿]