正交试验设计优化超声煎煮法提取丹参中丹参素的研究  

Optimization on extract technology of tanshinol natrium in Salvia Miltiorrhiza Bge with supersonicdecoct extraction method by orthogonal experimental design

作　　者：杨炳火[1] 夏崇才[1] 周芙琼[1] 胡新[1] 

机构地区：[1]南京市中医院药剂科,210001

出　　处：《环球中医药》2014年第12期926-928,共3页Global Traditional Chinese Medicine

基　　金：南京市卫生局科技发展项目(201108005)

摘　　要：目的探讨超声煎煮对丹参提取效果的影响,并筛选出最佳工艺。方法以丹参素含量为指标,对丹参进行超声煎煮正交实验,筛选出最佳提取工艺条件,并与煎煮提取法进行比较研究。结果超声时间和煎煮次数对试验结果有显著影响,而煎煮时间对结果无显著影响。最佳提取工艺为超声10分钟,煎煮20分钟,煎煮3次。结论所筛选出的最佳提取工艺合理、可行,在提取丹参中丹参素时,超声煎煮法优于煎煮法。Objective To study the effect of supersonic-decoct extraction method on the process of extracting Salvia miltiorrhiza Bge and get the optimum exracting condition. Methods With the content of tanshinol natrium as the parameters, gain better extracting method, then. take the way as using decoct ex-traction method to compare. Results Supersonic time and decoction frequency had the most notable effect on experimental results, while the decocting time had no notable effects. The optimum conditions are pres-ented as：the extraction time is 45 mins, the decocting time is 20 mins, and the decoction frequency is three. Conclusion The result from supersonic extraction is stable and reliable. It can be concluded that supersonic-decoct extraction method has evident effect on the extract technology of tanshinol natrium in Sal-via miltiorrhiza Bge.

关 键 词：超声煎煮法 丹参 丹参素 

分 类 号：R284.2[医药卫生—中药学]