一株拮抗姜瘟根际青枯假单胞杆菌的沙雷氏菌的分离与鉴定  

Isolation and Identification of a Serratia sp. Strain Against Ginger Bacterial wilt Caused by Pseudomonas solanacarum Smith

作　　者：李霞[1] 张小平[1] 李欣[2] 

机构地区：[1]中国科学院成都山地灾害与环境研究所,成都610041 [2]中国科学院近代物理研究所,兰州730000

出　　处：《湖北农业科学》2014年第20期4841-4844,共4页Hubei Agricultural Sciences

基　　金：中国科学院西部之光项目(Y006060XBO)

摘　　要：从生姜连作地土壤中分离到1株对生姜青枯假单胞杆菌(Pseudomonas solanacarum Smith)有强拮抗作用的沙雷氏菌株R11,对该菌株进行了形态特征、培养特征、生理生化和16S r DNA序列分析的系统鉴定,发现R11为革兰氏阴性菌,端生一根鞭毛。以16S r DNA序列为基础构建了包括11株相关种属细菌在内的系统发育树,R11与沙雷氏菌(Serratia sp.)十分接近,序列相似性值达99%。R11,isolated from soil of ginger in Sichuan Province,was effective in against ginger bacterial wilt caused by Pseudomonas solanacarum Smith.The morphological,cultural,physiological and biochemical characteristics,and 16S rDNA sequences were studied.R11 was a gram-negative bacillus with a flagellum on the top.A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the related bacteria species.In the phylogenetic tree,the overall similarity value between strain R11 and Serratia sp.was 99％.

关 键 词：沙雷氏菌(Serratia sp.) 16S RDNA 青枯假单胞杆菌(Pseudomonas solanacarum Smith) 

分 类 号：Q939[生物学—微生物学]