川贝母多倍体诱导条件的优化及含量测定  

Optimizing the Conditions of Inducing Polyploid of Fritillaria cirrhosa and Content Determination

作　　者：江明殊[1,2] 王跃华[2] 刘涛[2] 范文萍[2] 杨敏[2] 王晓蓉 

机构地区：[1]四川师范大学生命科学学院,成都610101 [2]成都大学生物产业学院,成都610106 [3]成都恩威投资集团有限公司,成都610200

出　　处：《湖北农业科学》2014年第20期4903-4907,共5页Hubei Agricultural Sciences

基　　金：成都市经济和信息化委员会资助的科研项目(2012);成都市科技计划项目(12GGYB413SW-001)

摘　　要：采用正交试验法考察秋水仙素浓度、浸泡时间、二甲基亚砜体积分数、处理温度对川贝母(Fritillaria cirrhosa)愈伤组织多倍体诱导的影响。以多倍体诱导率为指标,用正交试验表L9(34)进行试验,筛选最佳诱导条件。在考察的因素中,对川贝母多倍体诱导率影响的大小次序依次为秋水仙素浓度>浸泡时间>二甲基亚砜体积分数>处理温度。多倍体诱导条件的最优组合是秋水仙素浓度为900 mg/L、浸泡时间为48h、二甲基亚砜体积分数为4%、处理温度为20℃,在此条件下,其诱导率可达94.3%;细胞染色体鉴定结果为四倍体染色体数为2n=4x=48,诱导后的愈伤组织为多倍体;对野生、组培二倍体、组培多倍体川贝母鳞茎进行有效药用成分生物碱总含量测定,其中组培多倍体生物碱总含量最高为0.657%。The induction rate of optimized conditions of Fritillaria cirrhosa including the concentration of colchcine,the time of being soaked in colchcine,the volume fraction of dimethyl sulfoxide and temperature was determined by orthogonal text.A optimal combination was screened through the crossed test of L9（3^4）.The order of factors affecting the induction rate was the concentration of colchcine〉 the time of being soaked in colchcine〉 the volume fraction of dimethyl sulfoxide〉 temperature.The optimal combination was the concentration of colchcine 900 mg/L,the time of being soaked in colchcine 48 h,the volume fraction of dimethyl sulfoxide 4%,temperature 20 ℃,with induction rate of 94.3%.Compared with the chromosomal number,it was proved that the callus of F.cirrhosa was polyploidy.The contents of total bio-medicinal ingredients with wild,tissue culture of diploid and polyploid bulbs of F.cirrhosa were effectively determinated.The total alkaloid content of polyploid bulbs of F.cirrhosa was the highest with up to 0.657%.

关 键 词：川贝母(Fritillaria cirrhosa) 愈伤组织 多倍体 生物碱 正交试验 

分 类 号：Q813.12[生物学—生物工程]