芒(Miscanthus sinensis)再生体系的建立和优化  

Establishment and optimization of a regeneration system for Miscanthus sinensis

作　　者：黄丽芳[1] 殷绪明[1] 陈智勇[2] 肖亮[2] 易自力[2] 

机构地区：[1]中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室,湖南长沙410125 [2]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2014年第6期583-588,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家"863"课题(2011AA10020901);美国孟德尔生物技术公司合作项目(SR0373)

摘　　要：以芒(Miscanthus sinensis)的幼穗为材料,通过外植体消毒灭菌、愈伤组织诱导、不定芽分化增殖、生根和驯化移栽,建立芒组织培养的快速繁殖体系。结果表明:芒愈伤组织诱导的最佳培养基是MS+4.0 mg/L 2,4–D+1.0 mg/L 6–BA+0.5 mg/L Cu SO4,愈伤组织诱导频率达99.33%;不定芽分化和增殖的最佳培养基分别为MS+1.0mg/L 6–BA+0.4 mg/L NAA+0.4 mg/L IAA和MS+3.0 mg/L 6–BA+1.5 mg/L PP333+1.0 mg/L CCC,分化率为96%,增殖系数为9.1;生根培养基选用MS+0.5 mg/L IAA+0.5 mg/L NAA+0.5 mg/L CCC+0.5 mg/L PP333,生根率达100%;将再生苗移栽至用黄土和营养土配制的基质上,其成活率高达95%,且生长势良好。The rapid propagation system was established using the spike of Miscanthus sinensis as material through explants sterilization, adventitious bud differentiation and multiplication, rooting and transplanting acclimation. The results showed that the optimal medium for callus induction was MS＋ 4.0 mg/L 2,4-D ＋ 1.0 mg/L 6-BA ＋ 0.5 mg/L CuSO4, with which the induction frequency reached 99.33%. Best adventitious bud differentiation and multiplying medium was MS＋ 1.0 mg/L 6-BA ＋ 0.4 mg/L NAA ＋ 0.4 mg/L IAA and MS＋ 3.0 mg/L 6-BA ＋ 1.5 mg/L PP333 ＋ 1.0 mg/L CCC, with which the differentiation rate was 96% and the multiplying coefficient was 9.1. The best medium for rooting induction was MS＋ 0.5 mg/L IAA ＋ 0.5 mg/L NAA ＋ 0.5 mg/L CCC ＋0.5 mg/L PP333, with which the rooting rate was 100%. The regenerated plantlets grew well on the matrix consisting of loess and nutrition soil, and the survival rate was as high as 95%.

关 键 词：芒 能源植物 离体培养 正交设计 方差分析 

分 类 号：S543.9[农业科学—作物学]