转2–Cys Prx基因烟草F_1幼苗叶片光系统Ⅱ光化学活性对干旱胁迫的响应  

Response of PSⅡ photochemistry activity of F_1 progeny of transgenic tobacco seedlings with 2–Cys Prx to drought stress

作　　者：胡举伟[1] 张会慧[1,2] 逄好胜 孙广玉[1] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]黑龙江省科学院自然与生态研究所,黑龙江哈尔滨150040

出　　处：《湖南农业大学学报（自然科学版）》2014年第6期593-598,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：黑龙江省自然科学基金项目(ZD2011–05);哈尔滨市科技创新人才研究专项(2013RFXXJ063)

摘　　要：以转2–Cys Prx基因烟草(龙江911)为材料,测定和分析了干旱胁迫下转基因烟草的光化学活性。结果表明:干旱胁迫下,转2–Cys Prx基因烟草叶片OJIP曲线上K点和J点的相对荧光强度增加量明显低于非转基因烟草,而2 ms时有活性反应中心的开放程度(Ψo)明显高于非转基因烟草;增强2–Cys Prx基因的相对表达,增加了干旱胁迫下烟草叶片PSⅡ电子供体侧OEC的电荷分离能力和受体侧QA向QB的电子传递能力;转2–Cys Prx基因烟草叶片在干旱胁迫下,吸收光能用于QA–以后的电子传递的能量比例(φEo)明显大于非转基因烟草,而非光化学猝灭的最大量子产额(φDo)。2–Cys Prx基因的表达可以提高烟草幼苗叶片的光化学活性,并改变光能的分配来增强其抗旱能力。Transgenic tobacco plants with 2-Cys Prx （Longjiang911） was chosen to investigate the photochemical activity of transgenic tobacco plants under drought stress. The results showed that the relative fluorescence intensity in K and J point of OJIP curve in leaves of the transgenic tobacco plants with 2-Cys Prx were lower than those of the non transgenic tobacco （CK） under drought stress, but opening degree of surplus active reaction center （Ψo） at 2 ms was significantly higher than that of CK. Improved 2-Cys Prx expression could increase the photocatalytic activity of OEC in PS Ⅱelectron donor and the electron transport from QA to QB in acceptor side under drought stress. The electron transport chain beyond QA- （φEo） in leaves of the transgenic tobacco plants with 2-Cys Prx were higher compared to the CK, but best quantum yield of non-photochemical quenching （φDo） were less compared to the CK. 2-Cys Prx could increase drought-resistant ability of the transgenic tobacco plants by improving photochemical activity and changing absorbed light allocation in leaves of tobacco seedlings.

关 键 词：烟草 2-Cys Prx胁基因 干旱胁迫 叶绿素荧光动力学曲线 光反应 

分 类 号：S572.01[农业科学—烟草工业]