外源硝态氮对水培鸢尾叶片抗氧化酶活性的影响  

Effects of Nitrogen on the Antioxidant Enzyme Activity of Hydroponics Iris Leaves

作　　者：马杰[1] 赵兰枝[1] 刘弘[1] 

机构地区：[1]河南科技学院园艺园林学院,河南新乡453003

出　　处：《西北林学院学报》2014年第6期20-23,共4页Journal of Northwest Forestry University

基　　金：河南省重大科技攻关项目(092101310300)

摘　　要：以鸢尾为试验材料,采用营养液水培控制溶氧浓度的方法,研究了短期低氧胁迫下外源硝态氮对鸢尾体内的几个逆境指标的影响。NO-3浓度共设4个水平:0、7.5、15.0 mmol·L-1和22.5mmol·L-1,以1/2×Hoagland营养液正常低氧处理(NO-3浓度7.5mmol·L-1)为对照。结果表明:各处理SOD、POD、CAT酶活性均呈现先升高后降低的趋势。与对照相比,营养液加氮处理的SOD、POD、CAT酶活性升高,膜质过氧化程度减轻;氮浓度越大,酶活性升高幅度越大,而营养液缺氮处理的酶活性降低,膜质过氧化程度加重;营养液缺氮处理鸢尾体内的H2O2和MDA含量升高,而营养液加氮处理可降低O·-2的产生及MDA含量,且NO-3离子浓度高时效果更好,说明适当提高营养液中的氮素水平可以缓解低氧胁迫对鸢尾的伤害。Effects of exogenous application of nitrogen to nutrient solution on the activity of antioxidant enzymes under hypoxia stress were examined.The Iris plant were cultured in 1/2×Hogland nutrient solutions with different NO3^-concentrations（0,7.5,15.0,22.5mmol·L^-1）.Under the normal oxygen condition,7.5mmol·L^-1 NO3^-was used as the control.The results showed that the activities of SOD,POD and CAT of Iris leaves in the four treatments all increased at the first four days and then decreased.Compared with the control,the activities of SOD,POD and CAT of Irisleaves treated by 15.0or 22.5mmol·L^-1 NO3^-increased and the MDA content decreased.The contents of H2O2 and MDA of the treatment with nitrogen deficiency were the highest among the four treatments.Addition of nitrogen into the nutrient solution under hypoxia stress could decrease the contents of H2O2 and MDA.The above results suggested that exogenous nitrogen application in nutrient solution could antagonize the damage of hypoxia stress on Irisleaves.

关 键 词：硝态氮 酶活性 低氧胁迫 鸢尾叶片 

分 类 号：S718[农业科学—林学]