HIF-1α与COX-2在银屑病皮损中的表达及相关性研究  

Expression and correlation of HIF-1α and COX-2 in psoriatic lesions

作　　者：苏江维[1] 鞠小玲 汪洁[1] 余慧[1] 刘丽华[1] 张凤萍[1] 于春水[2] 

机构地区：[1]湖北省鄂州市中心医院皮肤科,436000 [2]四川省遂宁市中心医院皮肤科

出　　处：《中华临床医师杂志（电子版）》2014年第23期42-46,共5页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的检测低氧诱导因子-1α(HIF-1α)和环氧合酶-2(COX-2)在寻常型银屑病皮损及正常人皮肤组织中的表达水平,探讨HIF-1α和COX-2在银屑病发病机制中的作用。方法收集30例寻常型银屑病和20例正常人的临床资料以及皮损和皮肤组织。(1)用半定量RT-PCR检测银屑病皮损和正常皮肤组织中HIF-1α和COX-2 m RNA表达情况;(2)用Western blot检测银屑病皮损和正常皮肤组织中HIF-1α和COX-2蛋白表达情况。结果 HIF-1α和COX-2 m RNA相对表达量在寻常型银屑病组(1.32±0.04和1.21±0.04)的表达显著高于正常对照组(0.49±0.03和0.31±0.03),两组相比差异有统计学意义(P<0.05);且HIF-1αm RNA相对表达量与COX-2 m RNA相对表达量呈正相关(r=0.664,P<0.05);HIF-1α和COX-2蛋白在寻常型银屑病组(0.76±0.03和0.62±0.03)的表达显著高于正常对照组(0.44±0.05和0.28±0.06),两组相比差异均有统计学意义(P<0.05);且HIF-1α蛋白表达量与COX-2蛋白表达量呈正相关(r=0.601,P<0.05)。结论 HIF-1α和COX-2在银屑病皮损中异常高表达且呈正相关,可能在银屑病的发生发展过程中起着重要作用。ObjectiveTo study the expression and correlation of HIF-1αand COX-2 in samples of psoriasis vulgaris tissue and those of skin tissue which were from healthy individuals. To explore the role of HIF-1α and COX-2 in the pathogenesis of psoriasis.Methods 30 patients with psoriasis vulgaris and 20 normal human controls were enrolled into the study and obtained these lesions or normal skin tissues. （1）RT-PCR was used to examine the mRNA expressions of HIF-1α and COX-2 in samples of psoriasis vulgaris lesion tissues and those of skin tissues from healthy individuals. （2）Western blot was used to examine the protein expressions of HIF-1α and COX-2 in samples of psoriasis vulgaris lesion tissues and those of skin tissues from healthy individuals.Results The expressions of HIF-1α and COX-2 mRNA in psoriasis vulgaris tissues （1.32±0.04 and 1.21±0.04） were significantly higher than those in the normal group （0.49±0.03 and 0.31±0.03）. Which showed significant difference in the expressions of HIF-1α and COX-2 mRNA between the psoriasis and the control group （P〈0.05）. Moreover, The HIF-1αmRNA in psoriatic lesions was associated significantly with COX-2 mRNA （r=0.664,P〈0.05）. The expressions of HIF-1α and COX-2 protein in psoriasis vulgaris tissues （0.76±0.03 and 0.62±0.03） was significantly higher than those in the normal group （0.44±0.05 and 0.28±0.06）. Which showed significant different in the expressions of HIF-1α and COX-2 protein between the psoriasis and the control group（P〈0.05）; and HIF-1α protein in psoriatic lesions was associated significantly with COX-2 protein （r=0.601,P〈0.05）.ConclusionHIF-1α and COX-2 high expression and their positive correlation in psoriatic lesions might play an important role in genses and development of psoriasis.

关 键 词：银屑病 缺氧诱导因子1 Α亚基 环氧化酶2 逆转录聚合酶链反应 印迹法 蛋白质 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]