通心络对急性脑梗死大鼠脑组织及胎盘生长因子表达的影响  

Effects of Tongxinluo on the Pathologic Change and the Protein Expression of PlGF in Rats with Acute Cerebral Infarction

作　　者：盖聪[1] 李澎涛[2] 孙红梅[1] 张振强[3] 李卫红[1] 贾静[1] 于慧玲[1,4] 李聪[2] 

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学东直门医院,北京100007 [3]河南中医学院,河南郑州450046 [4]内蒙古医科大学,内蒙古呼和浩特010110

出　　处：《中医学报》2014年第11期1603-1607,共5页Acta Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(编号:2012CB518602);国家自然科学基金项目(编号:81273885)

摘　　要：目的:观察通心络对脑缺血模型大鼠脑组织病理学及胎盘生长因子(placental growth factor,PlGF)表达水平的影响,探讨其治疗脑梗死的作用机制。方法:以线栓法制作大鼠大脑中动脉缺血模型(MCAO),将SD大鼠随机分为假手术组、模型组、通心络组及丁苯酞组,分别在造模后1 d和3 d进行神经功能缺损评分,取脑和采集动脉血。采用HE染色方法观察脑组织的病理变化;免疫组织化学及酶联免疫(ELISA)的方法,分别检测脑组织和血清中PlGF的表达水平。结果:与模型组比较,通心络组能显著改善大鼠神经功能缺失症状,减轻大脑皮质及海马神经元的损伤,脑组织和血清PlGF表达均明显增强(P<0.05)。结论:通心络对急性脑梗死损伤具有保护作用,其作用机制可能与增加PlGF的表达有关。Objective：To observe effects of Tongxinluo（ TXL）on the pathologic change and the expression of placental growth factor in rats with acute cerebral infarction,and to explore its mechanism of action. Methods：Using filament method to copy middle cerebral artery occlusion（ MCAO）model of rats. The rats were randomly divided into 4 groups：the sham-operated group,the model group,TXL group and Butylphthalide group. After modeling for 1 day and 3 days,the neurological scores were taken to evaluated the rats,the morphologi-cal feature of neurons was observed by HE staining,the level of PlGF on the brain and serum were detected by immunohistochemistry and ELISA. Results：Compared with the model group,the neurological impairment of the TXL group were alleviated,cell injury of the hippocampus and cortex were relieved,brain and serum expression of PlGF were increased（P〈0. 05）. Conclusion：TXL has protective effects on rat cerebral ischemia brain injured by MCAO,which is related to regulating PlGF expression.

关 键 词：通心络 急性脑梗死 脑缺血 胎盘生长因子 

分 类 号：R285.5[医药卫生—中药学]