右美托咪定对内毒素血症大鼠急性肾损伤的保护作用  

Protective effect of dexmedetomidine on rats with acute renal injury induced by lipopolysaccharide

作　　者：于冬梅[1] 张铁铮[1] 周锦[1] 陈克研[1] 曹惠鹃[1] 

机构地区：[1]沈阳军区总医院麻醉科,沈阳110016

出　　处：《实用药物与临床》2014年第12期1545-1547,共3页Practical Pharmacy and Clinical Remedies

基　　金：辽宁省科技攻关项目(2012225006)

摘　　要：目的观察右美托咪定对内毒素血症大鼠急性肾损伤的影响。方法 18只雄性SD大鼠随机分为3组:对照组(C组)、内毒素组(L组)和右美托咪定组(D组),每组6只。C组持续泵注生理盐水;L组静脉注射脂多糖LPS 5 mg/kg;D组静脉注射LPS 5 mg/kg后,先给予负荷量的右美托咪定7μg/kg,15 min后以5μg/(kg·h)持续泵注。各组均观察6 h,取外周血和肾组织,采用酶联免疫吸附法检测血浆中肿瘤坏死因子-α(TNF-α)和IL-1β含量变化;采用自动生化分析仪检测血清肌酐(Scr)和尿素氮(BUN)含量;观察肾组织病理学变化。结果与C组相比,L组和D组的血清TNF-α、IL-1β、肌酐和尿素氮含量均显著升高(P<0.05),而D组的血清TNF-α、IL-1β、肌酐和尿素氮含量均显著低于L组(P<0.05);D组的肾组织病理学变化较L组有所减轻。结论右美托咪定能减轻脓毒血症引起的肾组织炎症反应,改善肾功能,具有一定的保护作用。Objective To observe the effects of dexmedetomidine on rats with acute renal injury induced by lipopolysaccharide.Methods 18 adult male SD rats were randomly divided into 3 groups：control group （group C,n =6）,LPS group （group L,n =6）,dexmedetomidine group （group D,n =6）.In group C and group L,the rats were infused with saline and lipopolysaccharide（5 mg/kg,iv）respectively.In group D,after the infusion of lipopolysaccharide （5 mg/kg,iv）,the rats were firstly infused the loaded dose of dexmedetomidine （7 μg/kg）,and then infused at a rate of 5 μg/（kg·h）.6 h later,3 mL blood was collected for measuring TNF-α,IL-1β,Scr and BUN.Histological analysis of the kidneys was performed.Results The levels of TNF-α,IL-1β,Scr and BUN in group L and group D were higher than those of group C （P ＜ 0.05）,and the above indexes in group D was lower than that of group L （P ＜ 0.05）.Histological changes：the kidney of the group D showed lessened injury.Conclusion Dexmedetomidine can alleviate the inflammation induced by lipopolysaccharide,improve the renal function and thus protect the kidneys from these injuries.

关 键 词：右美托咪定 内毒素 急性肾损伤 

分 类 号：R614[医药卫生—麻醉学]