‘长红枣’和‘金丝小枣’离体花药不定芽的诱导  

In vitro induction of adventitious buds from anther in Ziziphus jujuba Mill.'Changhongzao' and Z.jujuba Mill.'Jinsixiaozao'

作　　者：韩晶[1] 王玖瑞[2] 代丽[1] 刘颖[2] 肖京[1] 刘孟军[1] 

机构地区：[1]河北农业大学中国枣研究中心,河北保定071001 [2]河北农业大学林学院,河北保定071001

出　　处：《河北农业大学学报》2014年第6期38-42,共5页Journal of Hebei Agricultural University

基　　金：国家科技支撑计划(2013BAD14B03)

摘　　要：以2个主栽枣树品种‘长红枣’和‘金丝小枣’为材料,研究了基因型、基本培养基、植物生长调节剂及碳源对枣花药愈伤组织发生及不定芽分化的影响。结果表明：‘长红枣’较‘金丝小枣’的离体花药更容易诱导出愈伤组织。适合‘长红枣’花药愈伤组织诱导的生长调节剂组合为1.0mg/L 2,4-D＋2.0mg/L 6-BA和0.5mg/L NAA＋2.0mg/L 6-BA,出愈率均在94.00%以上。基本培养基MS与1/2MS对‘长红枣’花药愈伤组织诱导影响差异不显著,使用麦芽糖较蔗糖更适宜诱导花药愈伤组织。在MS＋6-BA 1.0~2.0 mg/L＋NAA 0.5mg/L培养基中,‘长红枣’花药愈伤组织的出芽率超过60.00%。‘金丝小枣’愈伤组织诱导和出芽培养基分别为1/2MS＋1.0mg/L 2,4-D和MS＋6-BA 1.0mg/L＋NAA 0.5mg/L,花药出愈率和愈伤出芽率分别达到56.17%和20.36%。The effects of genotype, basic medium, plant growth regulator and carbon source on callus induction from in vitro anther and adventitious bud formation were investigated using two major cultivars of Chinese jujube （Ziziphusjujuba Mill. ）,‘Changhongzao＇ and ＇Jinsixi- aozao＇. The results showed that the callus induction from anther was much easier in ‘Chang- hongzao＇ than in‘Jinsixiaozao＇. 1.0 mg/L 2,4-D ＋ 2.0 mg/L 6-BA and 0.5 mg/L NAA ＋ 2.0 mg/L 6-BA were appropriate plant growth regulator combinations for the anther callus induction in‘Changhongzao＇, in which the callus rate reached more than 94.00%. There was no significant difference of callus induction between MS and 1/2 MS basic medium in‘Chang- hongzao＇. The callus induction from anther was easier in medium supplemented with maltose than with sucrose. Adventitious bud rate of ‘Changhongzao＇ was more than 60.00% in medi- um MS ＋4- 6-BA 1.0N 2.0 mg/L ＋ NAA 0.5 mg/L. In case of‘Jinsixiaozao＇, the callus rate reached 56.17% in medium 1/2 MS ＋ 1.0 mg/L 2,4-D and the adventitious bud rate was 20.36% in medium MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.5 mg/L.

关 键 词：枣 花药 愈伤组织 不定芽 

分 类 号：S665.1[农业科学—果树学]