五味子藤茎中木脂素和多糖对小鼠急性肝损伤的保护作用  

Hepatoprotective effect of lignans and polysaccharides from the caulis of Schisandra chinensis against acute injury induced by CCl4in mice

作　　者：丁传波[1,2] 刘群 董岭[1] 赵婷[1] 李伟[1] 曹艳鹏 郑毅男[1] 刘文丛[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]珲春华瑞参业生物工程有限公司,吉林珲春130118 [3]沈阳纳尼亚生物医药科技有限公司,辽宁沈阳110117

出　　处：《华西药学杂志》2014年第6期648-650,共3页West China Journal of Pharmaceutical Sciences

基　　金：吉林省科技发展计划(20110904)

摘　　要：目的研究五味子藤茎中木脂素和多糖对CCl4诱导的小鼠急性肝损伤的保护作用。方法将72只ICR小鼠随机均分为正常对照组(0.9%生理盐水)、模型组(0.9%生理盐水)、联苯双酯组(150 mg·kg-1)、五味子藤茎木脂素高、中、低剂量组(200、100、50 mg·kg-1)、五味子藤茎多糖高、中、低剂量组(200、100、50 mg·kg-1),各组小鼠均ig给药7 d。除正常对照组外,其余各组于第7天给药结束后ip 10%CCl4。6 h后于眼球取血,离心后取血清,测定ALT、AST、TG;测定肝匀浆中SOD、MDA的含量;同时观察肝脏组织的病理学变化。结果与模型组的比较,木脂素高、中剂量组小鼠血清中的ALT、AST活性及TG水平均显著降低;多糖高剂量组的AST和TG水平也显著降低;木脂素和多糖均能显著升高肝脏中SOD的活性,降低肝脏中MDA的含量。结论五味子藤茎木脂素和多糖对CCl4诱导的小鼠急性肝损伤均有一定的保护作用。OBJECTIVE To observe the hepatoprotective effects of lignans and ploysaccharides from the caulis of Schisandra chinensis against acute injury induced by CCl4 in mice. METHODS 72 ICR mice were randomly divided into control group（ 0. 9% physiological saline）,model group（ 0. 9% physiological saline）,Biphenyldicarboxylate group（ 150 mg·kg- 1）,high,middle and low dose of lignans groups（ 200,100,50 mg·kg- 1）,and the high,middle and low dose of polysaccharides groups（ 200,100,50 mg·kg- 1）. All groups mice were ip 10% CCl4,except the control group,after the last treatment on the 7 day. After 6 h,the mice were sacrificed by bleeding and blood was collected to measure ALT,AST and TG. The clear supernatant from liver samples were used to determine the SOD and MDA. The pathological change of liver tissue was observed. RESULTS Compared with the model groups,high and middle dose lignans groups ALT,AST and TG levels decreased in serum. High dose groups of polysaccharides only reduced AST activity and the level of TG. Simultaneously,lignans and polysaccharides could increase SOD activity in the liver,the level of MDA in the liver was significantly decreased. CONCLUSION Lignans and polysaccharides from the caulis of Schisandra chinensis have hepatoprotective effect on acute injury induced by CCl4 in mice.

关 键 词：五味子藤茎 木脂素 多糖 肝损伤 

分 类 号：R96[医药卫生—药理学]