纳米SiO_2与介孔SiO_2对脐静脉血管内皮细胞毒性作用的比较研究  

Comparative Study on Silica Nanoparticles and Mesoporous Silica Induced Toxicity to Human Umbilical Vein Endothelial Cells

作　　者：卜德云[1] 牛照地[1] 田大年[1] 朱玲勤[1] 刘贺荣[1] 纪文武[1] 周健[1] 杨惠芳[1] 

机构地区：[1]宁夏医科大学公共卫生学院,宁夏750004

出　　处：《环境与职业医学》2014年第12期936-940,共5页Journal of Environmental and Occupational Medicine

基　　金：宁夏自然科学基金重点项目(编号:NZ13058);宁夏高等学校科学研究重点项目(编号:NGY2013057)

摘　　要：[目的]探讨并比较纳米Si O2(nano-Si O2)与介孔Si O2(SBA-15)对人脐静脉血管内皮细胞(HUVEC)的毒性效应及氧化损伤作用。[方法]将nano-Si O2、SBA-15按照不同作用浓度(0.00、6.25、12.50、25.00、50.00、100.00、200.00μg/m L)对HUVEC进行染毒,作用24 h后,测定HUVEC的增殖抑制情况、培养液中乳酸脱氢酶(LDH)的活性,及细胞内活性氧(ROS)的表达水平。[结果]与阴性对照组(0μg/m L)比较,nano-Si O2、SBA-15组细胞抑制率均升高(P<0.05),且具有明显的剂量-反应关系;低浓度(12.50μg/m L)时SBA-15组对细胞抑制率高于nano-Si O2组;随着染毒浓度增加,nano-Si O2组对细胞抑制率高于SBA-15组(P<0.05)。与阴性对照组比较,nano-Si O2、SBA-15组LDH、ROS水平均升高(P<0.05),且随着染毒浓度的升高呈现明显的剂量-反应关系(P<0.05);各剂量SBA-15染毒组的LDH和ROS水平均小于同剂量nano-Si O2染毒组(P<0.05)。[结论]nano-Si O2与SBA-15对血管内皮细胞均存在细胞毒性效应,氧化损伤可能是其细胞毒性机制之一。与nano-Si O2组相比,SBA-15细胞毒性较低。[ Objective ] To study and compare silica nanoparticles （nano-Si02） and mesoporous silica （SBA-15） induced cytotoxicity and oxidative damage on human umbilical vein endothelial ceils （HUVEC）. [ Methods ] HUVEC were exposed to nano-SiO2 and SBA-15 with different concentrations （0.00, 6.25, 12.50, 25.00, 50.00, 100.00, 200.00 μg/mL） for 24 hours. The inhibition rates of cell proliferation, expression levels of cell reactive oxygen species （ROS） and lactate dehydrogenase （LDH） activity in the medium supernatant were measured. [ Results ] Compared with the negative control group （0 μg/mL）, the inhibition rate of the nano-SiO2 group and the SBA-15 group were significantly increased （P 〈 0.05） and in an distinct dose-effect manner. At a low concentration of 12.50 μg/mL the inhibition rates of the SBA-15 group were higher than those of the nano-SiO2 group, the pattern reversed in high concentration range. Compared with the negative control group （0μg/mL）, the LDH activity and intracellular ROS levels of the nano-SiO： group or the SBA-15 group were significantly increased （P〈0.05）, also showing a distinct dose-effect relationship. The LDH activity and the ROS levels of the SBA-15 group at various doses were lower than those of the nano-SiO2 group at the same dose. [ Conclusion ] Both nano-SiO2 and SBA-15 impose toxic effects on HUVEC. Oxidative damage may be one mechanism of cytotoxicity induced by silica materials. SBA-15 shows lower cytotoxicity comparing with silica nanoparticles.

关 键 词：纳米SIO2 介孔SIO2 人脐静脉血管内皮细胞 细胞活力 乳酸脱氢酶 活性氧 

分 类 号：R114[医药卫生—卫生毒理学]