大孔树脂分离复方黑骨藤药物有效成分工艺的研究  

Separation of active ingredients from Compound Periploca forrestil by macroporous resin

作　　者：周秋月[1] 李琪[2] 李慧敏[2] 陈姚[1] 袁德彬[2] 张宏[2,3] 

机构地区：[1]四川师范大学化学与材料科学学院,四川成都610068 [2]四川师范大学生命科学学院,四川成都610101 [3]四川师范大学生命科学学院,四川成都610101 [4]四川师范大学植物资源应用与开发研究所,四川成都610101

出　　处：《中草药》2014年第21期3098-3104,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(31240087)

摘　　要：目的考察大孔树脂分离复方黑骨藤药物有效部位的工艺条件。方法以黑骨藤中杠柳苷、秦艽中龙胆苦苷和元胡中延胡索乙素为检测指标,采用HPLC法考察3种大孔树脂(D-101、AB-8、NKA-9)对杠柳苷、龙胆苦苷和延胡索乙素的吸附分离性能,并考察分离纯化杠柳苷、龙胆苦苷和延胡索乙素成分的最佳工艺条件。结果用大孔树脂D-101作为填料,样品与填料的比为1∶10,样品质量浓度为50 mg/m L,上样后吸附1 h,用60%乙醇为洗脱液,在p H 7.0条件下,20 BV洗脱1 h。结论该优选的工艺简便可行,大孔树脂D-101可作为分离纯化复方黑骨藤药物中杠柳苷、龙胆苦苷和延胡索乙素成分的有效方法。Objective To investigate the technology for the separation of the active ingredients from Compound Periploca forrestil （CPF） by macroporous resin. Methods The absorption and separation properties of three kinds of absorption resins （D-101, AB-8, and NKA-9） were studied and the separation and purification technological process of glucoperiplocymarin, gentiamarin, and tetrahydropalmatine from CPF were optimized by HPLC with glucoperiplocymarin in P. forrestil, gentiamarin in Gentiana macrophylla, and tetrahydropahnatine in Corydalisyanhusuo as indexes. Results Using macroporous resin D-101 as filler, the rate of sample and macroporous adsorption resin was 1 ＂ 10. The concentration of the sample was 50 mg/mL, adsorbing for 1 h and using 60% ethanol as eluent in the conditions of pH 7.0. The resin was washed by 20 BV in 1 h. Conclusion This technology is simple and feasible, and the process with macroporous resin D-101 is an effective method to separate and purify glucoperiplocymarin, gentiamarin, and tetrahydropalmatine from CPF.

关 键 词：大孔树脂 复方黑骨藤 杠柳苷 龙胆苦苷 延胡索乙素 分离工艺 

分 类 号：R284.2[医药卫生—中药学]