灵薏方多组分微乳的制备及其抗肺癌活性研究  

Preparation of Lingyi Formula multicomponent microemulsion and its anti-lung cancer activity

作　　者：袁成甜 贺俊杰[2] 陈彦[2] 瞿鼎[2] 孙文杰[2] 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]江苏省中医药研究院国家中医药管理局中药释药系统重点研究室,江苏南京210028

出　　处：《中草药》2014年第22期3284-3288,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81373979);江苏省中医药领军人才项目(LJ200913);江苏省自然科学基金资助项目(BK20141058)

摘　　要：目的制备灵薏方多组分微乳并对其抗肺癌活性进行研究。方法以灵芝多糖和薏苡仁多糖水溶液为水相,薏苡仁油为油相,荷载灵芝三萜,采用水滴定法制备灵薏方多组分微乳,测定其粒径、Zeta电位,并考察其稳定性。以Lewis荷瘤小鼠抑瘤率、体质量变化、免疫器官(肝、脾和胸腺)指数以及血浆中肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)水平为考察指标,结合肿瘤组织病理切片和TUNEL凋亡原位检测,评价灵薏方多组分微乳的抗肺癌活性。结果制备所得微乳形态圆整,平均粒径为(69.92±8.43)nm,多分散指数(PDI)为0.060±0.008,Zeta电位为(-11.30±1.34)m V。微乳组抑瘤率(57.25%)显著高于混悬液组(45.89%),免疫器官指数和血浆中TNF-α和IL-6水平显著升高,肿瘤组织病理切片和TUNEL凋亡原位检测显示微乳组抗肿瘤效果显著高于混悬液组。结论灵薏方多组分微乳具有较好的抗肺癌活性。Objective To prepare Lingyi Formula multicomponent microemulsion and evaluate its anti-lung cancer activity. Methods Lingyi Formula multicomponent microemulsion was prepared by aqueous titration method using polysaccharides solution of Ganoderma lucidum and Coix lachryrna-jobi var. ma-yuen as aqueous phase and coix seed oil as oil phase, loading ganoderma triterpenes. The average particle size, Zeta potential, and stability were detected. The results of ant^tumor efficacy including tumor inhibitory rate, body weight change, immune organ index, and concentration of TNF-α and IL-6 were investigated. Besides, pathological section of tumor tissue and TUNEL labeling were conducted subsequently. Results The prepared microenmlsion displayed spherical surface with mean droplet size of（69.92 ± 8.43） nm, polydispersity （PDI） of 0.060 ± 0.008, and Zeta potential of （-11.30± 1.34） mV. Tumor inhibitory rate of microemulsion （57.25%） was significantly higher than that of suspension （45.89%）, immune tissue index as well as the concentration of TNF-α and IL-6 were increased significantly. TUNEL labeling and pathological section of tumor tissue showed that the ant^tumor activity of microemulsion was significantly effective compared with that of suspensions. Conclusion Lingyi Formula multicomponent microemulsion has a good anti-lung cancer activity.

关 键 词：灵薏方 灵芝多糖 薏苡仁多糖 灵芝三萜 薏苡仁油 多组分微乳 抗肺癌 

分 类 号：R285.5[医药卫生—中药学]