雷帕霉素对人单核细胞来源的树突状细胞免疫功能的影响  

Effects of rapamycin on immune function of human monocyte-derived dendritic cells

作　　者：骆高江[1] 方明[1] 姜昌浩[1] 

机构地区：[1]义乌市中心医院心内科,322000

出　　处：《浙江医学》2014年第21期1771-1774,共4页Zhejiang Medical Journal

基　　金：义乌市科研计划项目(项目编号:12-3-26)

摘　　要：目的研究雷帕霉素对人树突状细胞(DC)免疫功能的影响。方法分离外周血单个核细胞,在含粒细胞巨噬细胞集落刺激因子(rhGM-CSF)、IL-4、胎牛血清及有或无雷帕霉素的培养条件下制备DC。用流式细胞仪检测DC表型(CD1a、CD83、CD86、HLA-DR);混合淋巴细胞反应(ML R)检测DC对同种异体T淋巴细胞的刺激能力;流式细胞仪检测DC吞噬功能和凋亡细胞数;ELISA法测定MLR上清液中的细胞因子。结果与对照组比较,经雷帕霉素处理的DC表面CD1a、CD83、CD86、HLA-DR的表达明显降低(均P<0.01);对T淋巴细胞刺激的能力下降(P<0.01);细胞吞噬能力下降(P<0.01);凋亡细胞数增加(P<0.01),MLR中细胞因子(TNF-α、IL-10、IL-12)浓度降低(均P<0.01)。结论雷帕霉素对人树突状细胞免疫功能有明显的抑制作用,可能是其防止冠状动脉支架术后再狭窄的机制之一。Objective To investigate the effects of rapamycin on immune function of human monocyte- derived dendritic cel s （DC）. Methods Human monocytes were isolated and cultured with fetal bovine serum, granulocyte- macrophage colony- stimulating factor （rhGM- CSF） and interleukin- 4 （rhIL- 4） with or without rapamycin. Al ogeneic T cel activation by DCs was tested by mixed lymphocyte reaction （MLR）. ELISA was used to detect the expression of IL- 10, IL- 12 and TNF- α. The im-munophenotype, cel apoptosis and endocytic activity of DCs were measured by flow cytometry. Results The level of CD1a, CD83, CD86 and HLA- DR in rapamycin treatment group was lower than those in control group（P〈0.01）. Rapamycin inhibited the endocytic and T- cel stimulating activity of DCs （P〈0.01）, induced DC apoptosis, and decreased the secretion of IL- 10, IL- 12 and TNF- a（P〈0.01）. Conclusion Rapamycin can inhibit the immune function of human monocyte- derived dendritic cel s, which may be one of the mechanisms for prevention of in- stent restenosis.

关 键 词：雷帕霉素 树突状细胞 免疫功能 

分 类 号：R392[医药卫生—免疫学]