栀子大黄汤血清药物化学的初步研究  

Preliminary study on serum pharmacochemistry of Zhizi Dahuang Decoction

作　　者：唐峥[1] 尹然[2] 毕开顺[2] 朱鹤云[2] 张晓书[2] 毛新娟[2] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中草药》2014年第23期3377-3382,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金青年科学基金项目(81202893);辽宁省教育厅科学研究一般项目(L2012360)

摘　　要：目的对栀子大黄汤(Zhizi Dahuang Decoction,ZDD)的入血成分进行分析研究,进而探讨其药效物质基础。方法采用血清药物化学研究方法,建立口服ZDD后大鼠血清的超快速液相色谱(UFLC)指纹图谱;通过比较ZDD、缺味方、单味药以及各组给药后所得血清样品,初步确定口服ZDD后大鼠血中移行成分及其来源生药。结果口服ZDD后在大鼠血中发现了21个入血成分,其中12个为代谢产物,9个为原型成分;经与对照品比对,指认了3个原型入血成分,分别为京尼平苷、芦荟大黄素和大黄酸。结论初步确定了ZDD的入血成分,为其药效物质基础的阐明奠定了基础。Objective To study the blood components in Zhizi Dahuang Decoction（ZDD） and to further explore its pharmacodynamic material basis. Methods UFLC fingerprints of serum in rats after ZDD treatment were established with serum pharmacochemistry method. The UFLC profiles of serum samples collected after the treatment of ZDD, ZDD without one of the component herbs and single crude herb were compared, and the transitional constituents to blood and their metabolites were analyzed. Results Twenty-one constituents absorbed into rat blood were detected after oral administration of ZDD, 12 of which were metabolites. Other nine were prototype constituents, three of which were recognized as geniposide, aloeemodin, and rhein by comparing with reference substances, respectively. Conclusion The constituents of ZDD absorbed into blood are preliminarily identified, which could lay the foundation for the illumination of its pharmacodynamic material basis.

关 键 词：栀子大黄汤 血清药物化学 入血成分 指纹图谱 物质基础 京尼平苷 芦荟大黄素 大黄酸 

分 类 号：R286.02[医药卫生—中药学]