黄皮树不同部位生长过程中主要生物碱成分的累积研究  

Accumulation of main alkaloids composition in growth process of different parts of Phellodendron chinense

作　　者：季可可 谢红旗[2] 蔡松 曾建国[1,2] 

机构地区：[1]湖南中医药大学湖南省植物功能成分利用协同创新中心,湖南长沙410208 [2]湖南农业大学国家中药材生产(湖南)技术中心,湖南长沙410128 [3]湖南湘泉药业股份有限公司,湖南吉首416000

出　　处：《中草药》2014年第23期3462-3466,共5页Chinese Traditional and Herbal Drugs

基　　金：国家科技支撑计划(2012BAI29B04);湖南省科技重大专项(2013FJ1011)

摘　　要：目的分析黄皮树不同生长树龄、不同采收季节、不同采收部位中小檗碱和黄柏碱的质量分数变化规律,为增加中药材种植收益提供依据。方法采用高效液相色谱分析方法对小檗碱和黄柏碱进行定量测定。结果黄皮树中小檗碱和黄柏碱的累积高低顺序均为根皮>茎皮>根;2种生物碱的量逐年变化,前3年量变化趋势明显,且第3年的茎皮量接近5%,显示"幼龄黄柏"有开发价值,第3年后2种生物碱量变化趋势减缓;同一树龄10月份采收的黄柏,其黄柏碱和小檗碱较高。结论小檗碱和黄柏碱代谢累积规律的分析和总结,为黄柏药材采收及资源的可持续利用提供了依据。Objective To analyze the content variation of phellodendrine and berberine from Phellodendron chinense in different years, different seasons, and different parts, and to provide the basis for increasing the planting benefit of Chinese herbal medicine. Methods High performance liquid chromatography（HPLC） analysis method was used to determine the contents. Results The contents of phellodendrine and berberine from different parts of P. chinense showed as the following order： root barks 〉 barks 〉 roots. The contents of the two kinds of alkaloids changed each year. In first three years, the content change trend was obvious, and the content in barks was close to 5% in the third year. The young P. chinense had development value. After the third year, the contents of the two kinds of alkaloids changed slowly. The contents of phellodendrine and berberine in October were higher. Conclusion The metabolic accumulation rules of berberine and phellodendrine are summarized, in order to provide the basis for the sustainable utilization of P. chinensis resources.

关 键 词：黄柏 小檗碱 黄柏碱 代谢累积 幼龄黄柏 

分 类 号：R282.21[医药卫生—中药学]