谷氨酰胺预处理对肝缺血-再灌注后肺损伤的干预效果及机制  

Protective effects of glutamine pretreatment on total hepatic ischemia/reperfusion induced lung injury

作　　者：刘荣[1] 罗振中[2] 

机构地区：[1]解放军第184医院麻醉科,江西鹰潭335000 [2]南昌大学第二附属医院麻醉科,江西南昌330006

出　　处：《临床军医杂志》2014年第12期1211-1214,共4页Clinical Journal of Medical Officers

摘　　要：目的观察谷氨酰胺预处理对肝缺血-再灌注后急性肺损伤的干预效果。方法成年SD雄性大鼠36只,按随机数字表法将其平均分为三组:假手术组(Sham,S组);缺血-再灌注组(I-R组);谷氨酰胺组(Gln,G组)。其中I-R组为完全阻断肝动脉、门静脉及胆总管(参照Pringle's法)持续缺血30 min后再灌注1 h;而G组以0.75 mg/kg谷氨酰胺于全肝血流阻断前60 min经尾静脉注射行预处理。测定肺组织肿瘤坏死因子-α(TNF-α)、核因子-κb(NF-κb)、髓过氧化物酶(MPO)活性;肺湿/干重比(W/D);HE染色观察肺组织形态学变化以及谷氨酰胺预处理对大鼠肝缺血-再灌注后肺组织热休克蛋白-70(HSP-70)表达的影响。结果与I-R组比较,G组大鼠肺组织W/D比、TNF-α、NF-κb水平明显下降,MPO活性显著降低,HSP-70蛋白表达水平明显增加(P<0.05),光镜下肺组织损伤明显改善。结论谷氨酰胺预处理可通过增加肺组织HSP-70表达,抑制NF-κb等炎症因子的释放,对肝脏缺血-再灌注后的急性肺损伤起到一定的保护作用。Abstract： Objective To observe the effect of pretreatment with glutamine on lung injury induced by intestinal ischemiafreperfu- sion in rats. Methods Thirty-six adult SD rats were randomized into 3 groups ： sham （S） group, hepatic ischemia/reperfusion （I/R） group and glutamine group. In the I/R group, total hepatic ischemia was accomplished by clipping portal vein and hepatic artery for 30 rain and the clips were then released, which was followed by one hour period of reperfusion; as for the G group, the animals were pretreated with glutamine （0.75mg/kg） 60 rain earlier before ischemia. Tumor necrosis factor-α （TNF-α） , nuclear factor-Kb （NF-Kb） , myeloperoxidase （MPO） and wet/dry ratios of lung tissues were detected. The changes in lung morphology were ob- served under light microscope, as well as the effects on the expression of heat shock protein-70 （HSP-70）. Results W/D ratio, TNF-α, NF-Kb and MPO activity in lung tissues after glutamine pretreatment were lower in the G group than in the I/R group, and the expression of HSP-70increased significantly（ P 〈 0.05 ）. The lung tissue damage of the G group became attenuated. Conclusion Pretreatment with glutamine plays a protective role in acute lung injury after hepatic ischemiafreperfusion in rats, and the en- hancement of HSP-70 expression is possibly one of the potential mechanisms.

关 键 词：谷氨酰胺 肝缺血-再灌注 急性肺损伤 热休克蛋白-70 

分 类 号：R563[医药卫生—呼吸系统]