人脐带沃顿胶干细胞对急性大鼠脊髓损伤的作用及脊髓组织中TNF-α和BDNF表达的影响  

Effects of Wharton's jelly cell transplant on acute spinal cord injury in rats and expressions of TNF-α and BDNF in spinal cord tissue

作　　者：渠瑞娜 马珊珊[1] 田毅[2] 姚宁[1] 崔渊博[1] 张文进[2] 韩康[1] 宋及时 杨波[2] 关方霞[1,2] 

机构地区：[1]郑州大学生命科学学院,郑州450001 [2]郑州大学第一附属医院,郑州450052

出　　处：《郑州大学学报（医学版）》2014年第6期765-769,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目81071008;U1404313;河南省杰出青年基金114100510005;河南省科技攻关项目122102310203;人事部留学回国人员择优资助项目

摘　　要：目的:探讨人脐带沃顿胶干细胞(WJCs)移植对急性脊髓损伤大鼠的治疗作用及其可能机制。方法:81只大鼠分为假手术组、模型组和WJCs移植组,每组27只。每组取6只,采用BBB评分法评估脊髓损伤后1、3、7、14、21和28 d各组大鼠的运动功能,采用透射电镜检测损伤局部超微结构并检测损伤后28 d脑源性神经营养因子(BDNF)的表达,其余大鼠采用实时荧光定量PCR和Western blot检测损伤后0、3、6、12、24、72、168 h损伤脊髓组织中肿瘤坏死因子(TNF-α)的表达。结果:脊髓损伤后模型组与WJCs移植组大鼠的运动功能均有不同程度的恢复,其中WJCs移植组较模型组恢复更明显。与模型组相比,WJCs移植组损伤区脊髓组织结构相对完整。模型组和WJCs移植组损伤脊髓组织中TNF-αmRNA和蛋白以及BDNF蛋白的表达较假手术组增加(P<0.05);与模型组相比,WJCs移植组中TNF-α的表达降低,而BDNF的表达增加(P<0.05)。结论:WJCs移植可改变脊髓损伤区的微环境,抑制损伤脊髓组织中TNF-α的表达,同时增加BDNF的表达,促进大鼠神经功能恢复,减少脊髓继发性损伤。Aim：To explore the effect of Wharton′s jelly cells （WJCs） transplant on the expressions of tumor necrosis factor alpha （TNF-α） and brain-derived neurotrophic factor （BDNF） in rats with acute spinal cord injury （SCI）, and to in-vestigate the function of WJCs in the inflammatory response and nerve repair after acute SCI .Methods：A total of 81 rats were allocated into sham operation group , model group and WJCs group （27 rats in each group）.In the WJCs group, 200μL WJCs cell suspension （about 1 ×106 cells） was injected by femoral vein at 10 min after the injury.Neurologic function was accessed by BBB score at 1 d, 3 d, 7 d, 14 d, 21 d and 28 d respectively after transplantation;the ultrastructures of injured spinal cord were detected by transmission electron microscopy;the mRNA and protein expressions of BDNF in the spinal cord tissue were detected by real-time quantitative PCR and Western blot , respectively at 28 d, and those of TNF-αlevel were detected at 0 h, 3 h,6 h, 12 h, 24 h, 72 h, 168 h after transplantation .Results：Neural function was recov-ered to some extent both in WJCs group and model group , and the former was much better .Compared with model group , the structure of SCI area of WJCs group was relatively intact .Compared with the sham operation group , the expressions of BDNF protein and TNF-αmRNA and protein were increased significantly both in model group and WJCs group （P〈0.05）;compared with model group, the expressions of TNF-α mRNA and protein decreased（P〈0.05）, while that of BDNF in-creased in the WJCs group（P〈0.05） .Conclusion：WJCs transplantation can change the microenvironment of the injured spinal cord by inhibiting the expression of TNF-αwhile promoting the expression of BDNF in the lesion , therefore reducing secondary spinal cord injury , which may be a potential mechanism underlying the recovery of motor function of injured rats .

关 键 词：人脐带沃顿胶干细胞 脊髓损伤 肿瘤坏死因子-α 脑源性神经营养因子 大鼠 

分 类 号：R681.54[医药卫生—骨科学]