不同来源冻融精子行卵胞浆内单精子注射的临床结局分析  

Clinical ICSI outcome with different sources of frozen-thawed sperm

作　　者：宋文妍[1] 赵万里[1] 杨庆岭[1] 孙莹璞[1] 姚桂东[1] 杨洪毅[1] 金海霞[1] 辛志敏[1] 

机构地区：[1]郑州大学第一附属医院生殖中心,郑州450052

出　　处：《郑州大学学报（医学版）》2014年第6期865-868,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：郑州市科技攻关项目340600531425

摘　　要：目的:探讨不同来源冻融精子行卵胞浆内单精子注射(ICSI)的效果。方法:回顾性分析因无精症采用睾丸和附睾精子行ICSI的895个周期,其中诊断性附睾活检冻融精子7个周期(A组),诊断性睾丸活检组织冻融11个周期(B组),附睾穿刺取精(PESA)行ICSI后剩余精子冻融13个周期(C组),睾丸穿刺取精(TESA)行ICSI后剩余精子冻融12个周期(D组),PESA新鲜精子630个周期(E组),TESA新鲜精子222个周期(F组)。结果:6组的平均MⅡ卵子数、平均优质胚胎数比较,差异均无统计学意义(F=0.326、1.513,P>0.05),优质胚胎率、种植率、临床妊娠率差异亦没有统计学意义(χ2=4.874、2.766、1.617,P>0.05)。结论:不同来源冻融精子行ICSI可以获得与新鲜精子相似的临床结局。Aim：To investigate the clinical outcome of intracytoplasmic sperm injection （ ICSI ） with different sources of frozen-thawed sperm .Methods：A total of 895 ICSI cycles were evaluated retrospectively .There were 7 cycles using fro-zen-thawed sperm after diagnostic puncture of epididymis , 11 cycles using frozen-thawed sperm after diagnostic puncture of testis, 13 cycles using frozen-thawed sperm from PESA after ICSI , 12 cycles using frozen-thawed sperm from TESA after ICSI, 630 cycles using the sperm from PESA and 222 cycles using the sperm from TESA .Results：The differences in the median numbers of MⅡ orum and high quality embtyo among the 6 groups had no statistical significance （ F =0.326, 1.513,P〉0.05）.Conclusion：The clinical outcome of ICSI with frozen-thawed testicular or epdidymal sperm is similar to that of ICSI with fresh epididymal or testicular sperm .

关 键 词：诊断性睾丸活检 诊断性附睾活检 冻融精子 卵胞浆内单精子注射 

分 类 号：R711.6[医药卫生—妇产科学]