孔雀石绿分子印迹表面等离子共振传感器的制备及分析应用  

Preparation of Surface Plasmon Resonance Sensor Based on Malachite Green Molecularly Imprinted Polymer and Its Analytical Application

作　　者：李洁[1] 陈长宝[1] 张丽丽[1] 周杰[1] 

机构地区：[1]山东农业大学化学与材料科学学院,山东泰安271018

出　　处：《分析科学学报》2014年第6期873-878,共6页Journal of Analytical Science

基　　金：国家自然科学基金(No.21175083)

摘　　要：利用表面等离子共振（SPR）光谱,结合分子印迹技术,制备了孔雀石绿分子印迹SPR传感器,建立了检测孔雀石绿的分析方法。探讨了pH值对分子印迹膜吸附特性的影响,并在最佳pH下对其吸附选择性进行了考察。研究结果表明,与相应非印迹传感膜相比,孔雀石绿印迹传感膜对孔雀石绿具有较高的吸附选择性能。该方法测定河水及河泥中孔雀绿的线性范围为8.0×10^-10 - 1.0×10^-8 mol/L,检出限（S/N=3）分别为8.83×10^-11 mol/L和1.55×10^-10 mol/L,平均回收率分别为91.97%和93.88%,相对标准偏差分别为1.2%和2.1%。该方法具有简单、快速、灵敏度高、重复性好等特点,适用于河水和河泥中孔雀石绿的测定。In this paper,a new SPR sensor for the detection of malachite green was prepared by combining the molecularly imprinted technique with surface plasmon resonance spectroscopy.The surface topography of the imprinted SPR sensor was undertaken by atomic force microscopy.The effect of pH value on the adsorption of the imprinted membrane was investigated.Meanwhile,we also evaluated the properties of its adsorptivity and selectivity at the optimal pH.Compared to the non-imprinted film,the imprinted film showed higher adsorption efficiency and selectivity for malachite green.The method showed good linearity between 8.0×10^-10 and 1.0×10^-8 mol/L.When the developed method was used to determine malachite green in the river water and river mud,the detection limits were 8.82×10^-11mol/L and 1.55×10^-10 mol/L based on a signal to noise ratio of 3.The mean recoveries were 91.97%and 93.88%,and the relative standard deviations（RSD）obtained were 1.2% and 2.1%,respectively.The results indicated that the developed SPR sensor for the detection of malachite green possessed the advantages such as simplicity,high-speed,high sensitivity and good recovery.

关 键 词：分子印迹聚合物膜 表面等离子共振 传感器 孔雀石绿 

分 类 号：O657[理学—分析化学]