高效液相色谱法快速测定烟丝水提取液中尼古丁和可天宁生物碱  

Fast Determination of Nicotine and Cotinine in Aqueous Extract of Cut Tobaccos by High Performance Liquid Chromatography

作　　者：孙金影[1] 吕思敏[1] 钟月春[1] 郑志明[1] 叶仲杰 崔燎[1] 吴铁[1] 

机构地区：[1]广东医学院药学院,广东东莞523808

出　　处：《分析科学学报》2014年第6期907-910,共4页Journal of Analytical Science

基　　金：广东医学院博士后启动基金(XB1123);广东医学院科技创新基金(STIF201112)

摘　　要：建立了烟丝水提取液中尼古丁和可天宁生物碱的高效液相色谱(HPLC)快速检测方法。色谱柱为ZORBAX Eclipse Plus C18柱,流动相为甲醇-0.02 mol/L KH2PO4-三乙胺(体积比10∶90∶0.05,pH=3.2)。将该方法用于不同品牌香烟烟丝中尼古丁和可天宁的含量分析,结果显示黄山、白沙、万宝路和广东湛江廉江烟丝厂烟丝中尼古丁生物碱含量分别为10.59±0.77 mg/g、13.53±0.38 mg/g、10.82±0.41mg/g和10.89±0.92mg/g;可天宁生物碱含量分别为0.53±0.04mg/g、0.38±0.02mg/g、0.24±0.01mg/g和0.51±0.001mg/g。A simple high performance liquid chromatography（HPLC）coupled with UV detection method was established for simultaneous analysis of nicotine（NIC）and cotinine（COT）in aqueous extract of cut tobaccos.A ZORBAX Eclipse Plus C18 column and mobile phase composed of CH3OH∶0.02 mol/L KH2PO4∶triethylamine=10∶90∶0.05%（adjusted to pH 3.2with H3PO4）were used for separation.The developed HPLC-UV method was used for nicotine and cotinine analysis in Huangshan,Baisha,Marlboro and Pipe tobaccos.Results showed that in the above real samples,nicotine content was 10.59±0.77mg/g,13.53±0.38mg/g,10.82±0.41mg/g and 10.89±0.92mg/g,and cotinine content was 0.53±0.04mg/g,0.38±0.02mg/g,0.24±0.01mg/g and 0.51±0.001mg/g,respectively.

关 键 词：高效液相色谱法 尼古丁 可天宁 烟草分析 

分 类 号：O657.72[理学—分析化学]