不同水杨酸处理方式对喜树幼苗铝胁迫的缓解效应  

Exogenous treatment with salicylic acid alleviates aluminum toxicity in Camptotheca acuminata seedlings

作　　者：王俊霖[1] 严晓茹 沈晓云[1] 赵梦婷[1] 张婷婷[1] 沈族桓 陈颖[1] 

机构地区：[1]南京林业大学生物与环境学院,南京210037

出　　处：《林业科技开发》2014年第6期54-58,共5页China Forestry Science and Technology

基　　金：国家级大学生实践创新计划项目(201310298003);江苏高校优势学科建设工程资助项目(PAPD);"十二五"国家科技支撑计划(2011BAD38B01)

摘　　要：通过根施和叶面喷施水杨酸(SA)的2种预处理方式,研究水培条件下水杨酸对喜树幼苗铝毒害的缓解效应。结果表明:1 000 mg/L的Al Cl3处理降低了喜树幼苗的单株鲜质量、主根长和侧根数量,叶绿素含量和根系活力也显著下降,细胞膜透性增加,说明喜树幼苗受到了一定程度的铝胁迫。铝处理下经2种不同方式水杨酸预处理的喜树幼苗在铝处理8 d和16 d后,都不同程度地提高了喜树幼苗的鲜质量、叶绿素含量、根系活力,降低了相对电导率,提高了喜树碱的含量,说明水杨酸能够有效缓解铝对喜树幼苗的伤害。从处理效果上看,50 mg/L SA叶面喷洒处理效果最好,25 mg/L SA的根施处理抑制了茎的伸长,但促进了侧根的形成及喜树碱的合成。The seedlings of Camptotheca acuminata were pre-immersed in Hoagland’s solution with 10,25 mg / L salicylic acid（ SA） or pre-sprayed leaves with 25,50 mg / L SA for three days,then were cultured in the presence of 1 000 mg / L Al Cl3（ SA ＋ Al） for 8 or 16 days. The seedlings exhibited a decline in fresh weight,main root length,number of lateral root,pigment content and root activity,but an increase in relative electric conductivity（ REC） under Al alone treatment.However,above mentioned parameters（ except REC） of seedlings were obviously up-regulated under Al treatment after pretreated with SA solution. The Al and SA ＋ Al treatments all increased the content of camptothecin in seedlings compared to control,but the content in treatments of SA ＋ Al was higher than that in Al alone treatment. The results showed that pretreatment with SA alleviated aluminum stress and enhanced aluminum tolerance in seedlings. The optimum treatment was 50 mg / L SA foliar spraying among four SA treatments. The treatment of 25 mg / L SA which immersed root inhibited the stem elongation,but it promoted the formation of the lateral roots and the synthesis of camptothecin in leaves and roots.

关 键 词：喜树 铝胁迫 水杨酸 幼苗 

分 类 号：S512.101[农业科学—作物学]