骨刺消巴布剂对大鼠急性软组织损伤模型的治疗作用及机理研究  

Therapeutic Effects and Mechanism of GuCiXiao Cataplasm for Rat Model with Acute Soft Tissue Injury

作　　者：魏国俊[1] 王志勇[1] 董林[1] 邢涛[1] 李盛华[1] 

机构地区：[1]甘肃省中医院,甘肃兰州730050

出　　处：《西部中医药》2014年第10期14-16,共3页Western Journal of Traditional Chinese Medicine

基　　金：兰州市科技局科技计划项目(编号2012-1-50)

摘　　要：目的:观察骨刺消巴布剂对大鼠急性软组织损伤模型组织内白介素-6(IL-6)与肿瘤坏死因子-α(TNF-α)含量的影响和对组织形态学的影响,探讨骨刺消巴布剂在治疗急性软组织损伤过程中的可能机制。方法:将36只SD大鼠随机分为4组,空白组6只,模型组10只,对照组10只,治疗组10只。重物打击法建立SD大鼠急性软组织损伤模型,分组采取相应处理。各组于第3天、第8天各处死一半,进行组织形态学观察和IL-6、TNF-α含量的测定。结果:模型组、对照组、治疗组样本内IL-6、TNF-α含量在伤后各时相均高于空白组(P<0.05),对照组、治疗组、空白组IL-6、TNF-α含量均低于模型组(P<0.05)。相应处理后第3天,治疗组与对照组样本内IL-6、TNF-α含量相比P<0.05;第8天组间比较P>0.05。治疗第3天后组织形态学病理程度分级对照组与治疗组相比P>0.05。但对照组、治疗组分别与模型组相比P<0.05。第8天时,治疗组与对照组组间相比P>0.05。结论:骨刺消巴布剂治疗急性软组织损伤是通过调节组织内IL-6、TNF-α的水平以抑制无菌性炎症过程而实现的,与奇正消痛贴相比,其治疗效果相当。Objective： To explore the potential mechanism of GuCiXiao cataplasm in treating acute soft tissue injury by observing the effects of GuCiXiao cataplasm on the contents of IL-6 and TNF-a in the rat model, as well as its influence on the histomorphology. Methods： All 36 SD rat were randomized into four groups, six rats in the blank group, ten rats in the model group, ten rats in the control group and ten rats in the treatment group. SD rat model with acute soft tissue injury was established by heavy objects punch method, corresponding treatment was given to different groups. The half of each group were sacrificed at the 3rd and 8th day to observe the histomorphology, the contents of IL-6 and TNF-α. Results： The model group, the control group and the treatment group were higher than the blank group in the contents of IL-6 and TNF-α after damaged in different periods （P〈0.05）, the control group, the treatment group and the blank group were lower than the model group in the contents of IL-6 and TNF-α （P〈0.05）. At the 3rd day after the corresponding treatment, there was significant difference in the comparison of the contents of IL-6 and TNF-α between the treatment group and the control group （P〈0.05）; there was insignficant difference in the comparison at the 8th day （P〉0.05）. Histological observation： At the 3rd day after treating, there was no significant difference in the comparison of histomorphology pathological changes classifcation between the control group and the treatment group （P〉0.05）. But there were significant differences in the comparison between the control group and the model group, the treatment group and the model group （P〈0.05）. There was no difference between the treatment group and the control group at the 8th day （P〉0.05）. Conclusion： GuCiXiao cataplasm in treating acute soft tissue injury is realized by regulating the levels of IL-6 and TNF-α to inhibit the process of aseptic inflammation, its effects are similar to QiZheng XiaoTong paste

关 键 词：骨刺消巴布剂 急性软组织损伤 白介素-6 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学]