α-硫辛酸对实验性变态反应性脑脊髓炎大鼠脊髓组织IL-17表达的影响  

Effect of alpha-lipoic acid on IL-17 expression in rats with experimental allergic encephalomyelitis

作　　者：徐晓臣[1] 赵冉冉[2] 徐飞[3] 李彦改[1] 陈朝旺[2] 檀国军[4] 

机构地区：[1]邯郸市第一医院神经内科,河北邯郸056002 [2]邯郸市第一医院急诊科,河北邯郸056002 [3]邯郸市第一医院泌尿科,河北邯郸056002 [4]河北医科大学第二医院神经内科,河北石家庄050071

出　　处：《海南医学》2014年第24期3589-3592,共4页Hainan Medical Journal

基　　金：河北省科技厅课题(编号:11276425)

摘　　要：目的研究α-硫辛酸(Alpha-lipoic acid,ALA)对实验性变态反应性脑脊髓炎(Experimental autoimmune encephalomyelitis,EAE)大鼠模型中脊髓组织IL-17表达的影响。方法健康雌性Wistar大鼠50只,随机分为正常对照组(NC组),EAE 7 d组(EAE-7 d组)、EAE 6个月病程组(EAE-6 m组)、硫辛酸急性期组(ALA-7 d组)和硫辛酸6个月病程组(ALA-6 m组)。HE染色观察病理学改变,免疫组织化学染色观察IL-17的表达变化。结果 HE染色结果显示,在α-硫辛酸治疗急性期组和6个月病程组,均可减轻脊髓组织的炎性反应和髓鞘损伤。免疫细胞化学染色结果显示,EAE-7 d组大鼠IL-17蛋白的表达比正常对照组增加(P<0.01);EAE-6 m组大鼠IL-17蛋白的表达比正常对照组增加(P<0.01);α-硫辛酸处理后急性期组和6个月病程组IL-17蛋白的表达明显降低(P<0.01)。结论α-硫辛酸在EAE大鼠模型中的保护作用可能是通过抑制IL-17的表达来实现的。Objective To explore the effects of alpha-lipoic acid （ALA） on IL-17 expression in rats with ex-perimental allergic encephalomyelitis （EAE）. Methods Fifty female Wistar rats were divided randomly into five groups： the normal control group （NC group）, EAE model group with 7 days （EAE-7 d group）, EAE model group with 6 months （EAE-6 m group）, ALA administrated EAE model group with 7 days （ALA treated EAE-7 d group）, ALA administrated EAE group with 6 months （ALA treated EAE-6 m group）. HE staining was used to detect the pathologic changes. Immunohistochemistry was used to detect the expression of IL-17. Results HE staining showed that, in alpha-lipoic acid treated groups （ALA treated EAE-7 d group, ALA treated EAE-6 m group）, inflammatory reac-tion and myelin damage were all relieved. According to immunohistochemistry, the expression of IL-17 in EAE-7 d group and in EAE-6 m group was significantly increased compared to NC group （P〈0.01）. However, the expression of IL-17 in alpha-lipoic acid treated groups significantly decreased compared with EAE-7 d group and EAE-6 m group re-spectively （P〈0.01）. Conclusion Alpha-lipoic acid may exert its neuroprotective effect through ameliorating inflamma-tive reacts.

关 键 词：多发性硬化 Α-硫辛酸 炎症 IL-17 

分 类 号：R332[医药卫生—人体生理学]