苍术苷抑制氧化磷酸化的作用机制研究  

Inhibitive Mechanism of Atractyloside for Oxidative Phosphorylation

作　　者：张文明[1] 杨桂蓉[1] 李弘烨 喻凯[1] 陈伟[1] 刘健[1] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031

出　　处：《中药新药与临床药理》2014年第6期660-663,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：中国科学院重点部署项目"中药(民族药)标准化研究"(KSZD-EW-Z-004);国家重点基础研究发展计划项目"973计划"项目(2009CB522804)

摘　　要：目的探讨苍术苷(ATR)抑制氧化磷酸化电子传递链可能的作用靶点。方法将制备好的线粒体悬液分为对照组、N-乙酰-L-半胱氨酸(NAC)组、鱼藤酮(ROT)组、NAC和ROT联合组,每组再分为4小组,分别用0,20,40,100滋mol·L-1的ATR处理。测定各组线粒体呼吸链中4种蛋白质复合体和ATP酶的活性。结果 ATR能够抑制复合体Ⅰ、Ⅳ和2种ATP酶的活性,且其抑制作用和剂量相关。结论 ATR可能影响呼吸链电子传递的始端复合体Ⅰ、末端状态复合体Ⅳ和ATP酶活性,最终抑制ATP的形成。Objective To research the possible targets of atractyloside for inhibiting respiratory chain of oxidative phosphorylation. Methods The mitochondria suspension was divided into 4 groups, namely blank control group,N-acetyl-L-cysteine（NAC）group, rotenone（ROT）group, and NAC-ROT combination group. And then each group was divided into 4 subgroups, which were treated with 0, 20, 40, 100 μmol·L-1 of atractyloside respectively. The activities of adenosine triphosphate（ATP） enzymes and 4 complexes at respiratory chain of each group were tested.Results Atractyloside could inhibit the activities of complex Ⅰ and Ⅳ at respiratory chain, and Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase. The effects were dose-dependent. Conclusion It suggested that atractyloside can inhibit the activities of complexⅠ at initial point and ATP enzymes and complex Ⅳ at end point of respiratory chain,which results into the inhibition of ATP production.

关 键 词：苍术苷 线粒体 氧化磷酸化 

分 类 号：R285.5[医药卫生—中药学]