青蒿素哌喹片对昆明种小鼠灌胃给药的急性毒性试验  

Acute Toxicity Test of Oral Administration of Artequick in KM mice

作　　者：徐勤[1] 李晓波[2] 刘婵[2] 袁征[2] 宋健平[1] 关业枝[2] 王琪[1] 

机构地区：[1]广州中医药大学,广东广州510006 [2]广州中医药大学科技产业园,广东广州510445

出　　处：《中药新药与临床药理》2014年第6期667-669,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省科技计划项目(2011B010500008);广东省产业技术与研究开发入库项目(2012B010300003)

摘　　要：目的研究青蒿素哌喹片（ATQ）对昆明种小鼠灌胃给药的急性毒性。方法按Bliss法设计试验,对小鼠单次灌胃ATQ,测定ATQ对小鼠半数致死量（LD50）和LD50的95%可信限,观察14 d内小鼠的毒性反应和死亡情况,对小鼠进行大体解剖,肉眼观察并进行组织病理学检查。结果 ATQ单次灌胃给药可使小鼠出现不同程度的毒性反应,包括自发活动减少、闭眼、俯卧、运动失调、震颤、惊跳、强制性-阵挛性抽搐、角弓反张。小鼠LD50为2802.38 mg·kg^-1,LD50的95%可信限为2343.51-3520.11 mg·kg^-1,死亡小鼠肉眼观察未发现各脏器异常。结论 ATQ对小鼠单次灌胃给药具有一定的毒性,涉及的毒性靶器官可能是中枢神经系统。Objective To study the acute toxicity of artequick after oral administration in mice. Methods By using the Bliss method, the LD50 and its 95 % confidence of artequick in mice were determined after single oral administration for evaluation of the toxicity within 14 days. The gross anatomy and histopathological examination for the dead mice were also carried out. Results After single oral administration, the mice showed various degrees of toxic reaction,showing as less spontaneous activities, closing eyes, face lying, incoordination, tremor, terror, forced paroxysmal spasm,and opisthotonus. The LD50 of artequick in mice was 2802.38 mg·kg^-1and its 95 % confidence was 2343.51-3520.11 mg·kg^-1. No abnormal alteration was shown in the organs of all the postmortem mice after the gross anatomy.Conclusion Single oral administration of Artequick has some toxic effects in mice,and the target of toxic organs may be involved the central nervous system.

关 键 词：青蒿素哌喹 急性毒性 小鼠 

分 类 号：R285.5[医药卫生—中药学]