王氏连朴饮对脾胃湿热证模型大鼠胃黏膜P53、Bcl-2和COX-2蛋白表达的影响  

Effect of Wang's Lian Pu Decoction on Protein Expression of P53, Bcl-2 and COX-2 in Gastric Mucosa of Rat Model with Splenogastric Damp-heat Syndrome

作　　者：黄琴[1] 王晶[1] 王和生[1] 王俊霞[1] 冯康[1] 谭芸[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《中药新药与临床药理》2014年第6期694-696,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：贵州省科技厅-贵阳中医学院联合基金资助项目(黔科合中药字[2012]LKZ7023)

摘　　要：目的观察王氏连朴饮对脾胃湿热证模型大鼠胃黏膜增殖与凋亡相关P53、Bcl-2和COX-2蛋白表达的影响,探讨该方治疗脾胃湿热证获效的可能作用机制。方法取SD大鼠50只,随机分为正常对照组,模型组,王氏连朴饮高、中、低剂量组(1.94,0.97,0.48 g·m L-1),共5组,每组10只。除正常组饲以普通饲料外,其余4组均以湿热环境加高脂高糖饮食和白酒综合法复制脾胃湿热证模型,连续灌胃给药7 d,观察该方对模型大鼠胃黏膜P53、Bcl-2及COX-2蛋白表达的影响。结果本实验P53、Bcl-2、COX-2基因蛋白表达定位于胞质,模型组大鼠表达明显升高,与正常对照组比较,有显著性差异(P<0.01)。王氏连朴饮高、中、低剂量组P53、Bcl-2及COX-2蛋白表达有一定程度下调,与模型组比较,具有显著性差异(P<0.01),尤以中剂量组作用最明显。结论王氏连朴饮可能通过下调脾胃湿热模型大鼠胃黏膜P53、Bcl-2及COX-2蛋白水平的表达,在一定程度上调节其胃黏膜细胞增殖与凋亡的失衡,进而达到对胃黏膜损伤的修复作用。Objective To observe the effect of Wang＇s Lian Pu Decoction（WLPD）on protein expression of proliferation and apoptosis correlated P53, Bcl-2, COX-2 genes in gastric mucosa of rat model wiht splenogastric damp-heat syndrome（SDHS）, and to explore its possible therapeutic mechanism for SDHS. Methods Fifty Sprague Dawley rats were evenly divided into normal control group, model group, and high-, middle- and low-dosage WLPD groups（1.94,0.97,0.48 g·m L^-1）. SDHS rat model was established by the combined method of damp-heat environment and feeding with high fat and sugar diet and wine. The intervention with gastric gavage of WLPD was given simultaneously together with the modeling. The treatment lasted 7 days,and the effects of WLPD on protein expression levels of P53,Bcl-2 and COX-2 genes were observed. Results The protein expression of p53, Bcl-2 and COX-2 genes was localized in cytoplasm and their expression levels were significantly increased in the model group as compared to normal control group（P〈0.01）. High-, middle- and low-dosage WLPD showed an effect on down-regulating the protein expression of P53, Bcl-2 and COX-2 genes to certain extent, and the middle-dose had the strongest effect, the difference being significant compared with the model group（P〈0.01）. Conclusion WLPD may down-regulate the protein expression of P53, Bcl-2 and COX-2 genes in gastric mucosa of SDHS rats, correct the imbalance of the proliferation and apoptosis of gastric mucosa cells,thus has protective effect on the gastric mucosa.

关 键 词：王氏连朴饮 脾胃湿热证 P53 Bcl-2 COX-2 大鼠 

分 类 号：R285.5[医药卫生—中药学]