金苓痛风舒微丸抗炎作用及其机制研究  

Experimental Study on Anti-inflammatory Effects of Jinling Tongfengshu Pellets and Its Mechanism

作　　者：王晓倩[1] 李鑫[1,2] 郭建生[1,3] 阳松威 

机构地区：[1]湖南中医药大学中药现代化研究重点实验室,湖南长沙410208 [2]湖南中医药大学中医诊断研究所,湖南长沙410208 [3]湖南中医药大学药学院,湖南长沙410208

出　　处：《中药新药与临床药理》2014年第6期700-704,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：2014年湖南省研究生科研创新项目(CX2014B350)

摘　　要：目的研究金苓痛风舒微丸的抗炎作用及其作用机制。方法采用二甲苯致小鼠耳廓肿胀及角叉菜胶致大鼠足肿胀实验探索金苓痛风舒微丸的抗炎作用。采用腺嘌呤、乙胺丁醇灌胃及关节腔注射尿酸钠复合造模的方法复制大鼠痛风性关节炎模型。将大鼠随机分为正常组,模型组,别嘌醇组(0.036 g·kg-1),痛风定组(0.432g·kg-1),金苓痛风舒微丸高、中、低剂量组(37.8,18.9,9.45 g·kg-1),共7组,每组10只。采用酶联免疫法(ELISA)检测血清中前炎症因子肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)及白介素-8(IL-8)的表达。并观察关节滑膜组织病理形态学的改变。结果金苓痛风舒微丸可有效抑制小鼠耳廓炎症肿胀(P<0.05)及大鼠足肿胀(P<0.05,P<0.01)。显著抑制痛风性关节炎大鼠血清中TNF-α、IFN-γ、IL-8的表达(P<0.05,P<0.01),减轻大鼠关节滑膜的炎性细胞浸润、充血、肿胀和坏死(P<0.05,P<0.01)。结论金苓痛风舒微丸对急性炎症具有显著的抗炎作用,其对痛风性关节炎的抗炎机制可能是通过抑制前炎症因子TNF-α、IFN-γ、IL-8的表达,进而调控滑膜组织中炎症反应链,从而发挥抗炎作用。Objective To investigate the anti-inflammatory effects and mechanism of Jinling Tongfe ngshu Pellets.Methods Xylene-induced mice ear edema test and carrageenan-induced rat paw edema test were carried out to explore the anti-inflammatory effects of Jinling Tongfe ngshu Pellets. Gouty arthritis model was induced with gastric gavage of adenine and ethambutol and by intra-articular injection of monosodium sodium urate（MSU）. Rats were randomized into normal control group, model group, allopurinol（0.036 g·kg^-1）group,Tongfeng Ding（0.432 g·kg^-1）group,and high-,medium- and low-dose Jinling Tongfe ngs hu Pellets groups（37.8,18.9,9.45 g·kg^-1）,10 rats in each group. After the last administration, enzyme-linked immunosorbent assay（ELISA） was used to detect the expression of proinflammatory cytokines of tumor necrosis factor alpha（TNF-α）, interferon gamma（IFN-γ） and interleukin-8（IL-8） in the serum. The pathological changes of synovial tissue were observed. Results Jinling Tongfe ngs hu Pellets could effectively inhibit inflammation in mice ear swelling（P〈 0.05）and rat paw edema（P〈 0.05,P〈 0.01）,significantly inhibit the expression of TNF-α,IFN-γ and IL-8（P〈 0.05,P〈 0.01） in the serum of gouty arthritis rats, and relieve pathological changes oft synovial inflammatory cell infiltration, congestion, swelling and tissue necrosis of the joint synovium（P〈 0.05,P〈 0.01）. Conclusion Jinling Tongfe ngshu Pellets can significantly inhibit acute inflammation which induced by xylene and carrageenan. And its anti-inflammatory mechanism may be related with the anti-inflammatory effects through inhibiting the expression of proinflammatory cytokines of TNF-α,IFN-γ and IL-8,and regulating synovial tissue inflammatory response chain.

关 键 词：金苓痛风舒微丸 痛风性关节炎 抗炎作用 作用机制 

分 类 号：R285.5[医药卫生—中药学]