尿酸和嘌呤在水和磷酸盐缓冲介质中溶解度的测定  

Determination of Solubility of Uric Acid and Purine in Water and Phosphate Buffer

作　　者：程莉[1] 张加玲[1] 

机构地区：[1]山西医科大学公共卫生学院卫生化学教研室,太原030001

出　　处：《中国食物与营养》2014年第11期67-69,共3页Food and Nutrition in China

基　　金：山西省自然科学基金"食物中嘌呤的检测方法及应用研究"(项目编号:2010011054-1)

摘　　要：目的:测定尿酸和4种嘌呤(鸟嘌呤、次黄嘌呤、黄嘌呤、腺嘌呤)在水和磷酸盐缓冲介质中的溶解度,以期为嘌呤在体内的代谢及其终产物尿酸对心血管作用的研究提供参考。方法:在室温20℃下,将上述5种物质分别溶于蒸馏水及磷酸盐缓冲液(0.05 mol/L,p H=7.40)中制成饱和溶液,采用紫外分光光度法分别测定其浓度。结果:在水中的溶解度由高至低依次为腺嘌呤(66.15 mg/100 m L)、次黄嘌呤(39.95 mg/100 m L)、尿酸(6.23mg/100 m Ll)、黄嘌呤(1.14 mg/100 m L)和鸟嘌呤(0.17 mg/100 m L);在磷酸盐缓冲液中溶解度由高至低依次为尿酸(194.54 mg/100 m L)、腺嘌呤(65.22 mg/100 m L)、次黄嘌呤(29.27 mg/100 m L)、黄嘌呤(2.27 mg/100m L)和鸟嘌呤(<0.1 mg/100 m Ll)。结论:尿酸在磷酸盐缓冲液中的溶解度远远高于水中,是水中的32倍;腺嘌呤、次黄嘌呤和黄嘌呤在2种介质中的溶解度相近,鸟嘌呤在2种介质中均最难溶,甚至在磷酸盐介质中几乎不溶。【Objective】To determination the solubility of uric acid and purine including guanine,hypoxanthine,xanthine and adenine in the water and phosphate buffer to provide reference for study on metabolism of purine in vivo and effect of uric acid on cardiovascular.【Method】Saturated solution of 5 kinds of substances were made by dissolving them into distilled water and phosphate buffer（0.05 mol/L,p H = 7.40） respectively,then the concentrations of solutions were determined by Ultraviolet-visible Sepectrophotometry.【Result】The solubilities of 5 kinds of substances in distilled water were in order of adenine（66.15 mg/100 m L）,hypoxanthine（39.95 mg/100 m L）,uric acid（6.23 mg/100 m L）,xanthine（1.14 mg/100 m L） and guanine（0.17 mg/100 m L）.In the phosphate buffer,solubilities were in order of uric acid（194.54 mg/100 m L）,adenine（65.22 mg/100 m L）,hypoxanthine（29.27 mg/100 m L）,xanthine（2.27 mg/100 m L）and guanine（less than 0.1 mg/100 m L） respectively.【Conclusion】The solubility of uric acid in the phosphate buffer was much higher（32times） than distilled water.The solubilities of adenine,hypoxanthine and xanthine were separately approximate in both water and phosphate buffer.Guanine was hardly dissolved in both solvent medium,even undissolved in the phosphate buffer.

关 键 词：尿酸 嘌呤 溶解度 紫外分光光度法 

分 类 号：R446.1[医药卫生—诊断学]