激光熔融静电纺丝法制备PLLA/PCL/nHA复合纤维支架及细胞相容性评价  

Preparation and cell-compatibility of PLLA/PCL/nHA Composite scaffolds prepared by laser melt-electrospinning

作　　者：王娇娜[1,2] 王哲[1] 李秀艳[1] 李从举[1,2] 

机构地区：[1]北京服装学院材料科学与工程学院,北京100029 [2]北京市服装材料研究开发与评价重点实验室,北京100029

出　　处：《化工新型材料》2014年第12期81-84,共4页New Chemical Materials

基　　金：北京市属高校创新能力提升计划项目(TJSHG201310012021);国家自然科学基金项目(51073005;21274006);北京市自然科学基金项目(2112013);北京市属高等学校人才强教深化计划(IHLB);国家973项目(2010CB933501);北京市教委科技发展计划重点项目(KZ201210012013);北京服装学院一般科研项目(2012A-09)

摘　　要：利用激光熔融静电纺丝技术制备了PLLA/PCL及PLLA/PCL/nHA复合纤维,热压后形成层压复合纤维支架。利用扫描电镜对纤维支架进行了表征,同时对其进行了亲水性的测试,最后通过倒置荧光显微镜和MTT实验对复合纤维支架的细胞相容性进行了评价。研究结果表明,层压复合纤维支架的直径和孔结构具有多样性,nHA能够提高PLLA/PCL层压纤维支架的亲水性,改善支架的细胞相容性,增加细胞的附着能力,提高细胞的存活率。Poly-L-lactide/poly caprolactone（PLLA/PCL）and Poly-L-lactide/poly caprolactone/nano-hydroxyapatite（PLLA/PCL/nHA）composite nanofibers were prepared via laser melt-elect rospinning technique.The morphology and hydrophilicity of the electrospun fibers were characterized by using scanning electron microscopy and contact angle experiment.Human embryo kidney cells（HEK 293cells）were seeded on melt electrospun scaffolds and the cell growth situation was evaluated by inverted fluorescence microscope and MTT test.The results indicated that the diameters and pore structures of the electrospun fibers are of the variety.The nHA particles can improve the hydrophilicity and cell-compatibility of composite scaffolds.

关 键 词：PLLA PCL 激光熔融静电纺丝 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]